Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) 抗体 [EPR13673] (ab180124)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673]
Heterogeneous Nuclear Ribonucleoprotein (A1-like) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Jurkat, K562, A431 and 293 cell lysates; Human cervix squamous cell carcinoma tissue; K562 and 293 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13673 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/5000 - 1/10000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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特記事項 |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000 - 1/10000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250. |
ターゲット情報
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関連性
The function remains unknown. -
参照データベース
- Entrez Gene: 144983 Human
- SwissProt: Q5TBS2 Human
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別名
- HNRPA1L antibody
- LOC144983 antibody
- MGC102957 antibody
- RP11-78J21.1 antibody
画像
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Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
Immunofluorescent analysis of 293 (paraformaldehyde-fixed cells, 4%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/250 dilution and counterstained with DAPI (blue).
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Flow Cytometry (Intracellular) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
Intracellular flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like)with ab180124 at 1/10 dilution or a rabbit IgG (negative), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab180124 は 1 報の論文で使用されています。
- Lim J et al. The histone variant H3.3 G34W substitution in giant cell tumor of the bone link chromatin and RNA processing. Sci Rep 7:13459 (2017). PubMed: 29044188