Anti-HESX1 抗体 (ab67728)
Key features and details
- Mouse polyclonal to HESX1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-HESX1 antibody
HESX1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to HESX1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein within Human HESX1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- HESX1 transfected lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67728の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
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機能
Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation. -
関連疾患
Septooptic dysplasia
Growth hormone deficiency with pituitary anomalies
Pituitary hormone deficiency, combined, 5 -
配列類似性
Belongs to the ANF homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Strongly expressed in Rathke pouch in seven-week-old embryo. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- ANF antibody
- CPHD5 antibody
- hAnf antibody
see all
画像
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Western blot - Anti-HESX1 antibody (ab67728)All lanes : Anti-HESX1 antibody (ab67728) at 1/500 dilution
Lane 1 : HESX1 transfected lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab67728 は 1 報の論文で使用されています。
- Takagi M et al. A novel mutation in HESX1 causes combined pituitary hormone deficiency without septo optic dysplasia phenotypes. Endocr J 63:405-10 (2016). PubMed: 26781211
