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Anti-HES5 抗体 (ab25374)

Reviews (3)Q&A (2)References (19)
Immunohistochemistry (Frozen sections) - Anti-HES5 antibody (ab25374)

    Key features and details

    • Rabbit polyclonal to HES5
    • Suitable for: WB, ELISA, IHC-Fr
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-HES5 antibody [EPR15578] (ab194111)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-HES5 antibody
      HES5 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to HES5

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WB, ELISA, IHC-Frmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide corresponding to Mouse HES5 aa 50-150.
      Database link: P70120

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab25374の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    IHC-Fr (1)
    1/200 - 1/1000.

    This product has not been tested with paraffin sections.
    特記事項
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    IHC-Fr
    1/200 - 1/1000.

    This product has not been tested with paraffin sections.

    ターゲット情報

    • 機能

      Transcriptional repressor of genes that require a bHLH protein for their transcription.

    • 組織特異性

      Expressed in fetal heart and brain tumors.

    • 配列類似性

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.

    • ドメイン

      Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
      The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • bHLHb38 antibody
      • Class B basic helix-loop-helix protein 38 antibody
      • Hairy and enhancer of split 5 antibody
      • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
      • HES 5 antibody
      • hes family bHLH transcription factor 5 antibody
      • Hes5 antibody
      • HES5 protein antibody
      • HES5_HUMAN antibody
      • MGI:104876 antibody
      • Transcription factor HES-5 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-HES5 antibody (ab25374)
      Immunohistochemistry (Frozen sections) - Anti-HES5 antibody (ab25374)

      ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr.  The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes.  A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes.  HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.

    参考文献 (19)

    ab25374 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab25374 は 19 報の論文で使用されています。

    • Du Z  et al. SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway. Biomed Res Int 2022:9235837 (2022). PubMed: 36246971
    • Gao Q  et al. Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia. Neural Regen Res 16:1252-1257 (2021). PubMed: 33318402
    • Morrugares R  et al. Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2. Cell Mol Life Sci 77:2621-2639 (2020). PubMed: 31605148
    • Fan J  et al. Targeting the Notch and TGF-ß signaling pathways to prevent retinal fibrosis in vitro and in vivo. Theranostics 10:7956-7973 (2020). PubMed: 32724452
    • Huang H  et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). PubMed: 31323391
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HES5 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (differentiated induced pluripotent stem cells)
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Specification
    differentiated induced pluripotent stem cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 19 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-HES5 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse brain)
    Specification
    mouse brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 26 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HES5 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Neural cells)
    Specification
    Neural cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 24 2010

    Question

    Dear Abcam technical service,



    I would like to ask to you some detailed information about your anti HES5 antibody(ab25374).

    I've read that it has been tested for Western blot.

    Do you have the protocol for Western blot that you've used for testing it?



    Thank you in advance



    Giuseppe

    ----

    Read More
    Answer

    Thank you for your inquiry.

    I am happy to confirm that ab25374 is tested and guaranteed for WB with mouse and human samples.

    It was tested using standard WB procedure which can be found here:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11375

    https://www.abcam.com/index.html?pageconfig=resource&rid=11414

    https://www.abcam.com/index.html?pageconfig=resource&rid=12128

    https://www.abcam.com/index.html?pageconfig=resource&rid=13045

    I also would like to suggest to use a positive control like mouse embryonic cortex.

    I hope this information is helpful and wish you a good weekend.

    Read More

    Question

    Ab25374-this is an ab against nuclear protein. On the datasheet, in the IF staining figure, it seems cytopasmic. Please check and advise.

    Read More
    Answer

    The Uniprot localization of Hes5 is listed as Nuclear (by similarity) to other transcriptional repressors. However, Hes5 is widely expressed in the developing nervous system (Akazawa et al., 1992) and in embryos Hes5 is ubiquitously expressed inside the CNS, especially in undifferentiated cells (http://www.gensat.org/imagenavigator.jsp?imageID=15026). The image shows IF of e14.5 mouse embryo and displays both nuclear and cytoplasmic staining for ab25374. I hope this clarifies the matter.

    Read More

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