Anti-HES5 抗体 (ab25374)
Key features and details
- Rabbit polyclonal to HES5
- Suitable for: WB, ELISA, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HES5 antibody
HES5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HES5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse HES5 aa 50-150.
Database link: P70120 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Constituents: PBS, 40% Glycerol -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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|
ELISA |
Use at an assay dependent concentration.
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|
IHC-Fr | (1) |
1/200 - 1/1000.
This product has not been tested with paraffin sections. |
特記事項 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
1/200 - 1/1000. This product has not been tested with paraffin sections. |
ターゲット情報
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機能
Transcriptional repressor of genes that require a bHLH protein for their transcription. -
組織特異性
Expressed in fetal heart and brain tumors. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
ドメイン
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
see all -
別名
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
画像
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ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr. The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes. A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes. HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab25374 は 23 報の論文で使用されています。
- Hu HJ et al. Effects of hyperbaric oxygen on Notch signaling pathway after severe carbon monoxide poisoning in mice. Med Gas Res 13:23-28 (2023). PubMed: 35946219
- Zeng W et al. Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization. Exp Ther Med 25:278 (2023). PubMed: 37206572
- Du Z et al. SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway. Biomed Res Int 2022:9235837 (2022). PubMed: 36246971
- Wang S et al. Functional Cooperation of α-Synuclein and Tau Is Essential for Proper Corticogenesis. J Neurosci 42:7031-7046 (2022). PubMed: 35906071
- Gao Q et al. Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia. Neural Regen Res 16:1252-1257 (2021). PubMed: 33318402