Anti-Hepcidin-25 抗体 [EPR18074] (ab187778)
![Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)](https://www.abcam.co.jp/ps/products/187/ab187778/Images/ab187778-531274-anti-hepcidin-25-antibody-epr18074-western-blot.jpg "Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18074] to Hepcidin-25
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hepcidin-25 antibody [EPR18074]
Hepcidin-25 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18074] to Hepcidin-25 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human Hepcidin fragment protein; Human plasma, serum and liver lysate; Rat serum treated with LPS.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18074 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187778の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa).
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa). |
ターゲット情報
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機能
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317). -
組織特異性
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. -
関連疾患
Hemochromatosis 2B -
配列類似性
Belongs to the hepcidin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 57817 Human
- Entrez Gene: 84604 Rat
- Omim: 606464 Human
- SwissProt: P81172 Human
- SwissProt: Q99MH3 Rat
- Unigene: 8821 Human
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製品の状態
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure -
別名
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
see all
画像
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Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1 : Serum from rat treated with LPS at 30 µg
Lane 2 : Serum from rat treated with LPS at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST
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![Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)](https://www.abcam.co.jp/ps/products/187/ab187778/Images/ab187778-258231-anti-hepcidin-25-antibody-epr18074-western-blot.jpg)
Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution + Human Hepcidin fragment protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
In house recombinant Human Hepcidin is a His-tagged fragment protein (aa25-84).
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![Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)](https://www.abcam.co.jp/ps/products/187/ab187778/Images/ab187778-258227-anti-hepcidin-25-antibody-epr18074-western-blot.jpg)
Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Observed band size: 3 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
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![Anti-Hepcidin-25 antibody [EPR18074] (ab187778)](https://www.abcam.co.jp/ps/products/187/ab187778/Images/ab187778-5-benefits-of-recombinant-antibodies.png)
Anti-Hepcidin-25 antibody [EPR18074] (ab187778)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab187778 は 3 報の論文で使用されています。
- Nazemian V et al. Anti-hyperalgesia effect of nanchelating based nano particle, RAc1, can be mediated via liver hepcidin expression modulation during persistent inflammation. Int Immunopharmacol 69:337-346 (2019). PubMed: 30776642
- Liu B et al. Reduced hepcidin level features osteoporosis. Exp Ther Med 16:1963-1967 (2018). PubMed: 30186425
- Jover B et al. Sodium restriction modulates innate immunity and prevents cardiac remodeling in a rat model of metabolic syndrome. Biochim Biophys Acta Mol Basis Dis 1863:1568-1574 (2017). PubMed: 28254494