Anti-Hepatitis B Virus Core Antigen 抗体 [C1] (ab8637)
Key features and details
- Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
- Suitable for: ELISA, IP, WB, ICC/IF
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
製品の概要
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製品名
Anti-Hepatitis B Virus Core Antigen antibody [C1]
Hepatitis B Virus Core Antigen 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen -
由来種
Mouse -
特異性
This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
Ab8637 was raised against serotype ayw but will work with all other genotypes.
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アプリケーション
適用あり: ELISA, IP, WB, ICC/IFmore details -
種交差性
交差種: Hepatitis B virus -
免疫原
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus Core Antigen. Purified Hepatitis B Core Antigen
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エピトープ
Around aa positions 70-80, major, or "c1" determinant -
特記事項
This product was raised against native assembled HBV core (HBV capsid, or particulated core).
For denaturing western blots and denaturing Immunofluorescence we would recommend one of the following:
Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639)
Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)
These products are raised to the same, but denatured, HBcAg protein sequence thus have more robust activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
C1 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8637の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000.
We recommend boiling (100°C) the sample for 10 minutes immeditaley before loading and using a 2x or 4x Laemmli sample buffer (contains SDS). A 2X concentrated Laemmli buffer is: 4% SDS, 10% beta-mercaeptoethanol, 20% glycerol, 0.1M Tris pH 6.8, and 0.005% of bromophenol blue. |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/1000. We recommend boiling (100°C) the sample for 10 minutes immeditaley before loading and using a 2x or 4x Laemmli sample buffer (contains SDS). A 2X concentrated Laemmli buffer is: 4% SDS, 10% beta-mercaeptoethanol, 20% glycerol, 0.1M Tris pH 6.8, and 0.005% of bromophenol blue. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. -
細胞内局在
Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus. -
参照データベース
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944568 Hepatitis B virus
- SwissProt: P03146 Hepatitis B virus
- SwissProt: P03147 Hepatitis B virus
- SwissProt: P03148 Hepatitis B virus
- SwissProt: P0C573 Hepatitis B virus
- SwissProt: P69706 Hepatitis B virus
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別名
- C antibody
- Capsid protein antibody
- Core and e antigen antibody
see all
画像
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Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg (full length, 1-183)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (23)
ab8637 は 23 報の論文で使用されています。
- Li YY et al. Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation. Cell Mol Gastroenterol Hepatol 15:533-558 (2023). PubMed: 36270602
- Zuo D et al. A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo. Protein Cell 14:37-50 (2023). PubMed: 36726760
- Zhang W et al. Notoginsenoside R1 inhibits hepatitis B virus replication by modulating SIRT1 activity. Acta Virol 67:51-58 (2023). PubMed: 36950885
- Zhuo H et al. RP11-40C6.2 Inactivates Hippo Signaling by Attenuating YAP1 Ubiquitylation in Hepatitis B Virus-associated Hepatocellular Carcinoma. J Clin Transl Hepatol 11:323-333 (2023). PubMed: 36643034
- Zheng Y et al. Canocapavir Is a Novel Capsid Assembly Modulator Inducing a Conformational Change of the Linker Region of HBV Core Protein. Viruses 15:N/A (2023). PubMed: 37243280