Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A74] (ab23418)
Key features and details
- Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2/Perlecan
- Suitable for: ICC/IF, WB, IP, ELISA, IHC-Fr, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]
Heparan Sulfate Proteoglycan 2/Perlecan 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2/Perlecan -
由来種
Mouse -
特異性
ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). -
アプリケーション
適用あり: ICC/IF, WB, IP, ELISA, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Cow, Human -
免疫原
Tissue, cells or virus corresponding to Cow Heparan Sulfate Proteoglycan 2/Perlecan.
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エピトープ
Epitope is located in domain V of Perlecan. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
A74 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23418の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration.
|
| IP |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| AP |
Use at an assay dependent concentration.
|
|
| IHC-P | (3) |
Use at an assay dependent concentration. PubMed: 22278369
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
AP
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 22278369 |
ターゲット情報
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機能
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. -
組織特異性
Found in the basement membranes. -
関連疾患
Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. -
配列類似性
Contains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain. -
翻訳後修飾
Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 444872 Cow
- Entrez Gene: 3339 Human
- Omim: 142461 Human
- SwissProt: P98160 Human
- Unigene: 562227 Human
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別名
- Basement membrane specific heparan sulfate proteoglycan core protein antibody
- Endorepellin (domain V region) antibody
- Heparan sulfate proteoglycan of basement membrane antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab23418 は 17 報の論文で使用されています。
- Teoh CW et al. The loss of glycocalyx integrity impairs complement factor H binding and contributes to cyclosporine-induced endothelial cell injury. Front Med (Lausanne) 10:891513 (2023). PubMed: 36860338
- Zhang H et al. Exosome Derived from Mesenchymal Stem Cells Alleviates Hypertrophic Scar by Inhibiting the Fibroblasts via TNFSF-13/HSPG2 Signaling Pathway. Int J Nanomedicine 18:7047-7063 (2023). PubMed: 38046235
- Ng CY et al. Macrophages bind LDL using heparan sulfate and the perlecan protein core. J Biol Chem 296:100520 (2021). PubMed: 33684447
- Nelson V et al. Angiogenic Secretion Profile of Valvular Interstitial Cells Varies With Cellular Sex and Phenotype. Front Cardiovasc Med 8:736303 (2021). PubMed: 34527715
- Horton ER et al. Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2. Adv Healthc Mater 9:e1901669 (2020). PubMed: 32129003