AB26265

Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A76]

Name: Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A76] (ab26265) | Abcam
Brand: Abcam Limited
SKU: AB26265
Price: 73000 JPY
Availability: InStock

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]

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(8 Publications)

Mouse Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for IP, ELISA, WB, IHC-P, AP, IHC-Fr and reacts with Human, Cow, Sheep samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing Heparan Sulfate Proteoglycan 2/Perlecan protein.

別名を表示する

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

A76

アイソタイプ

IgG1

キャリアフリー

交差種

Cow, Human, Sheep

アプリケーション

IHC-P, ELISA, WB, IHC-Fr, IP, AP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody is highly specific for Heparan Sulfate Proteoglycan 2/Perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "AP" : {"fullname" : "Affinity Purification", "shortname":"AP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Protein-A/G purified.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.

Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (PubMed : 12435733, PubMed : 15591058, PubMed : 19789387). In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity).. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.

See full target information Basement membrane-specific heparan sulfate proteoglycan core protein

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Biofabrication 13: PubMed33910175

2021

A perfusable vascularized full-thickness skin model for potential topical and systemic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Sacha Salameh,Nicolas Tissot,Kevin Cache,Joaquim Lima,Itaru Suzuki,Paulo André Marinho,Maité Rielland,Jérémie Soeur,Shoji Takeuchi,Stéphane Germain,Lionel Breton

Life (Basel, Switzerland) 11: PubMed33668945

2021

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Beyer,Anna Blocki,Matthew Chung Yin Cheung,Zoe Ho Ying Wan,Babak Mehrjou,Roger Dale Kamm

Journal of molecular histology 50:285-294 PubMed30993430

2019

Type XI collagen-perlecan-HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Smith,James Melrose

Development (Cambridge, England) 145: PubMed29180569

2018

The skeletal phenotype of achondrogenesis type 1A is caused exclusively by cartilage defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ian M Bird,Susie H Kim,Devin K Schweppe,Joana Caetano-Lopes,Alexander G Robling,Julia F Charles,Steven P Gygi,Matthew L Warman,Patrick J Smits

Biomaterials 67:354-64 PubMed26241755

2015

3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel S Puperi,Liezl R Balaoing,Ronan W O'Connell,Jennifer L West,K Jane Grande-Allen

Histochemistry and cell biology 134:251-63 PubMed20690028

2010

Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker.

Applications

IHC-P, IHC-P

Species

Sheep, Human

Susan M Smith,Cindy Shu,James Melrose

Matrix biology : journal of the International Society for Matrix Biology 29:63-73 PubMed19788922

2009

Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan.

Applications

WB, ELISA

Species

Human, Human

Martin D Rees,John M Whitelock,Ernst Malle,Christine Y Chuang,Renato V Iozzo,Anastasia Nilasaroya,Michael J Davies

Biotechnic & histochemistry : official publication of the Biological Stain Commission 83:47-53 PubMed18568676

2008

The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus.

Applications

IHC-P

Species

Sheep

J Melrose,S M Smith,M M Smith,C B Little

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A76]

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

Abcam product promise