JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB23418

Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A74]

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

4

(6 Reviews)

|

(20 Publications)

Mouse Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF, AP, IHC-Fr and reacts with Human, Cow samples. Cited in 20 publications. Immunogen corresponding to Cell preparation containing Heparan Sulfate Proteoglycan 2/Perlecan protein.

別名を表示する

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

A74

アイソタイプ

IgG1

キャリアフリー

No

交差種

Cow, Human

アプリケーション

ICC/IF, IHC-P, ELISA, AP, WB, IHC-Fr, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

Epitope is located in domain V of Perlecan.

特異性

ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

style
left-column

代替製品

Primary Antibodies

AB2501

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]

Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (AB2501)

  • 5
  • 10
Primary Antibodies

AB26265

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]

  • 0
  • 0
Primary Antibodies

AB255829

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] (AB255829)

  • 0
  • 0

組合せ製品

Assay Kits

AB274393

Human HSPG ELISA Kit (Perlecan)

Recombinant|SimpleStep

Sandwich ELISA - Human HSPG ELISA Kit (Perlecan) (AB274393)

  • 0
  • 0
Primary Antibodies

AB6586

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

  • 5
  • 123
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "AP" : {"fullname" : "Affinity Purification", "shortname":"AP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.
Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (PubMed : 12435733, PubMed : 15591058, PubMed : 19789387). In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity).. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.
See full target information Basement membrane-specific heparan sulfate proteoglycan core protein
style
left-column

文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70103 PubMed40189804

2025

Structural changes in cerebral microvasculature induced by ferroptosis contribute to blood-brain barrier disruption in Alzheimer's disease: an autopsy study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Cao,Mei-Ying Huang,Chen-Hui Mao,Xue Wang,Yuan-Yuan Xu,Xiao-Jing Qian,Chao Ma,Wen-Ying Qiu,Yi-Cheng Zhu

International journal of nanomedicine 18:7047-7063 PubMed38046235

2023

Exosome Derived from Mesenchymal Stem Cells Alleviates Hypertrophic Scar by Inhibiting the Fibroblasts via TNFSF-13/HSPG2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Zhang,Chengyu Zang,Wen Zhao,Linfeng Zhang,Rui Liu,Zhang Feng,Jie Wu,Rongtao Cui

Frontiers in medicine 10:891513 PubMed36860338

2023

The loss of glycocalyx integrity impairs complement factor H binding and contributes to cyclosporine-induced endothelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chia Wei Teoh,Magdalena Riedl Khursigara,Carolina G Ortiz-Sandoval,Jee Woo Park,Jun Li,Arlette Bohorquez-Hernandez,Valentina Bruno,Emily E Bowen,Spencer A Freeman,Lisa A Robinson,Christoph Licht

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

Frontiers in cardiovascular medicine 8:736303 PubMed34527715

2021

Angiogenic Secretion Profile of Valvular Interstitial Cells Varies With Cellular Sex and Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Nelson,Vaidehi Patil,LaTonya R Simon,Kelsey Schmidt,Chloe M McCoy,Kristyn S Masters

The Journal of biological chemistry 296:100520 PubMed33684447

2021

Macrophages bind LDL using heparan sulfate and the perlecan protein core.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,John M Whitelock,Helen Williams,Ha Na Kim,Heather J Medbury,Megan S Lord

Advanced healthcare materials 9:e1901669 PubMed32129003

2020

Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2.

Applications

Unspecified application

Species

Unspecified reactive species

Edward R Horton,Queralt Vallmajo-Martin,Ivan Martin,Jess G Snedeker,Martin Ehrbar,Ulrich Blache

Nature communications 10:3656 PubMed31409793

2019

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Astgik Petrosyan,Paolo Cravedi,Valentina Villani,Andrea Angeletti,Joaquin Manrique,Alessandra Renieri,Roger E De Filippo,Laura Perin,Stefano Da Sacco

Journal of tissue engineering and regenerative medicine 13:1362-1374 PubMed31067012

2019

Influence of fibrin matrices and their released factors on epidermal substitute phenotype and engraftment.

Applications

Unspecified application

Species

Unspecified reactive species

Maia M Alexaline,Brice Magne,Amparo Zuleta Rodríguez,Muriel Nivet,Daniel Bacqueville,Jean-Jacques Lataillade,Marina Trouillas

Frontiers in bioengineering and biotechnology 6:156 PubMed30410879

2018

Microvascular Networks From Endothelial Cells and Mesenchymal Stromal Cells From Adipose Tissue and Bone Marrow: A Comparison.

Applications

Unspecified application

Species

Unspecified reactive species

Karoline Pill,Johanna Melke,Severin Mühleder,Marianne Pultar,Sabrina Rohringer,Eleni Priglinger,Heinz R Redl,Sandra Hofmann,Wolfgang Holnthoner
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com