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リコンビナントRabMAb

Anti-HEPACAM 抗体 [EPR25296-150] (ab300571)

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Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
  • Anti-HEPACAM antibody [EPR25296-150] (AB300571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25296-150] to HEPACAM
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HEPACAM antibody [EPR25296-150]
    HEPACAM 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25296-150] to HEPACAM

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: Untreated HepG2 (human hepatocellular carcinoma epithelial cell), HepG2 (human hepatocellular carcinoma epithelial cell). Human, mouse and rat brain tissue lysates. IHC-P: Human, mouse, and rat cerebrum.

  • 特記事項

     ab300571 does not react in IHC-Fr with mouse, flow cytometry with human and mouse, and immunoprecipitation with human. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300571の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000.
特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.

  • 配列類似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • ドメイン

    The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ25530 antibody
      • GlialCAM antibody
      • HECAM_HUMAN antibody
      • HEPACAM antibody
      • Hepatocyte cell adhesion molecule antibody
      • Protein hepaCAM antibody
      see all

    画像

    • Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      All lanes : Anti-HEPACAM antibody [EPR25296-150] (ab300571) at 1/1000 dilution

      Lane 1 : HepG2 whole cell lysate treated with Protein Deglycosylation Mix II
      Lane 2 : Untreated HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate, 20
      Lane 3 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20
      Lane 4 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Performed under non-reducing conditions.

      Observed band size: 46 kDa why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control:LNCaP(PMID:29658567).

      HEPACAM is a glycoprotein(PMID: 15885354) of approximately 46-72 kDa and detected as a  46 kDa band after treated with Protein Deglycosylation MIX II. 

      The samples are unboiled.

       Exposure time: 103 seconds.

       

    • Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      All lanes : Anti-HEPACAM antibody [EPR25296-150] (ab300571) at 1/2000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Performed under non-reducing conditions.

      Observed band size: 46-72 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      Blocking and diluting buffer and concentration:5% NFDM/TBST. 

      The samples are unboiled.

      Exposure time: 81 seconds. 

       

    • Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Western blot - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Anti-HEPACAM antibody [EPR25296-150] (ab300571) at 1/2000 dilution + Human brain tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 2000 cells

      Observed band size: 46-72 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 15 seconds. 

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HEPACAM with ab300571 at 1/500 dilution (0.994 µg/mL), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Cytoplasmic staining on human cerebrum. The section was incubated with ab300571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HEPACAM with ab300571 at 1/500 dilution (0.994 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Cytoplasmic staining on mouse cerebrum is observed. The section was incubated with ab300571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HEPACAM with ab300571 at 1/500 dilution (0.994 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab300571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEPACAM antibody [EPR25296-150] (AB300571)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling HEPACAM with ab300571 at 1/500 dilution (0.994 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: No staining on human kidney is observed. The section was incubated with ab300571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Anti-HEPACAM antibody [EPR25296-150] (AB300571)
      Anti-HEPACAM antibody [EPR25296-150] (AB300571)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300571 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300571 は論文での使用が確認できていません。

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