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AB259528

Anti-Hemojuvelin 抗体 [EPR21836-266] - BSA and Azide free (Detector)

Anti-Hemojuvelin antibody [EPR21836-266] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Hemojuvelin antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

別名を表示する

Hfe2, Rgmc, Hjv, Hemojuvelin, Hemochromatosis type 2 protein homolog, Hemojuvelin BMP coreceptor, RGM domain family member C

1 Images
Sandwich ELISA - Anti-Hemojuvelin antibody [EPR21836-266] - BSA and Azide free (Detector) (AB259528)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Hemojuvelin antibody [EPR21836-266] - BSA and Azide free (Detector) (AB259528)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243685).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21836-266

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB243685

Mouse RGM-C ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)

  • 0
  • 0
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
Primary Antibodies

AB103296

Anti-DHCR7 antibody

Western blot - Anti-DHCR7 antibody (AB103296)

  • 0
  • 0
Primary Antibodies

AB318178

Alexa Fluor® 488 Anti-CD18 antibody [C71/16]

Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD18 antibody [C71/16] (AB318178)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab259528 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse RGM-C. The recommended pair for sandwich ELISA is:
Capture: ab259527, Mouse RGM-C Capture Antibody (unconjugated)
Detector: ab259528, Mouse RGM-C Detector Antibody (unconjugated)
The reference range value is 46.88 - 3000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Hemojuvelin also known as HJV or RGMc is a glycosylphosphatidylinositol-anchored protein that plays a significant role in iron homeostasis. It has a molecular mass of about 42 kDa. Expression of hemojuvelin is mainly observed in the liver heart and skeletal muscles. This protein is essential for maintaining systemic iron levels and regulates how much iron is absorbed and stored in the body.
Biological function summary

Hemojuvelin interacts with other proteins to modulate iron metabolism. It acts as a coreceptor for bone morphogenetic proteins (BMPs) enhancing BMP signaling which is critical in regulating the peptide hormone hepcidin. Hemojuvelin itself is not part of a larger complex but it works closely with BMPs to control hepcidin expression. Hepcidin is the key hormone that dictates iron absorption and its release from macrophages maintaining iron balance.

Pathways

Hemojuvelin functions primarily within the BMP signaling and hepcidin regulatory pathways. The interaction with BMP6 is particularly critical. The BMP6-hemojuvelin complex boosts the SMAD signaling cascade resulting in increased hepcidin expression. This cascade impacts iron levels in the body by limiting intestinal iron absorption and controlling iron recycling by macrophages.

Hemojuvelin mutations can lead to juvenile hemochromatosis a condition characterized by excessive iron buildup in the body. This protein's dysfunction can severely affect iron homeostasis leading to detrimental health outcomes. Moreover its connection to the regulation of hepcidin links hemojuvelin to conditions like anemia of chronic disease where hepcidin expression is aberrantly increased or decreased. Understanding the interaction of hemojuvelin and hepcidin provides insights into the mechanisms behind these iron-related disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.
See full target information Hjv
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Abcam product promise

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