AB131225

Anti-Hemoglobin subunit beta + Hemoglobin subunit delta 抗体 [EPR8322(B)]

Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal Hemoglobin subunit beta/ba1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications.

別名を表示する

Hemoglobin subunit delta, Delta-globin, Hemoglobin delta chain, HBD, Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

All lanes:

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human red blood cell (RBC) lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

false

Supplier Data

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Blocking/Dilutiing buffer and concentration : 5% NFDM/TBST

Exposure time : 100 seconds

All lanes:

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution

Lane 1:

Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (DDDDK tag N-Term + His tag N-Term) (<a href='/products/proteins-peptides/recombinant-human-hemoglobin-subunit-alpha-protein-denatured-ab131697'>ab131697</a>) at 0.1 µg

Lane 2:

His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg

Lane 3:

His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg

Lane 4:

His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg

Lane 5:

His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg

Lane 6:

His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg

Lane 7:

His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg

Lane 8:

Western blot - Recombinant Human Hemoglobin subunit zeta protein (His tag C-Terminus) (<a href='/products/proteins-peptides/recombinant-human-hemoglobin-subunit-zeta-protein-ab95347'>ab95347</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8322(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin subunit delta also known as Hb delta or delta hemoglobin is a component of the hemoglobin protein family which primarily includes hemoglobin subunit beta (Hb beta). Hemoglobin delta has a molecular mass of approximately 16 kDa. It expresses mainly in erythroid cells where it functions as part of the hemoglobin A2 (HbA2) molecule. HbA2 is a minor hemoglobin variant which includes two alpha globin chains and two delta globin chains. Delta hemoglobin plays a role in oxygen transport in red blood cells albeit to a lesser extent than the major form hemoglobin A (HbA) which contains hemoglobin subunit beta.

Biological function summary

Hemoglobin subunit delta participates in oxygen binding and transport. As part of the hemoglobin A2 complex it facilitates the pickup and release of oxygen molecules in tissues and organs. Although HbA2 only constitutes about 2-3% of total hemoglobin in adults it serves as an important backup system for effective oxygen delivery. The delta subunit closely interacts with partners like the alpha globin chains to perform its role in the hemoglobin complex.

Pathways

Hemoglobin subunit delta participates in the erythropoiesis pathway which is critical for red blood cell development. It works alongside proteins such as adult hemoglobin forms and alpha-like globins to ensure proper tissue oxygenation. The hypoxia-inducible factor pathway also involves hemoglobin components adjusting erythropoiesis in response to low oxygen levels though the direct role of delta hemoglobin in this adaptive process is comparatively minor.

Hemoglobin subunit delta has a connection with beta-thalassemia. This disorder results from mutations affecting beta globin synthesis leading to insufficient hemoglobin function. As a result HbA2 levels often rise as a compensatory mechanism. Sickle cell anemia mainly linked to mutations in the beta globin gene also exhibits some compensatory shifts in globin expression although delta hemoglobin itself is not directly involved. These disorders highlight the interconnected role of different hemoglobin chains including delta hemoglobin in red blood cell pathophysiology.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Involved in oxygen transport from the lung to the various peripheral tissues.

See full target information Hemoglobin subunit delta

追加のターゲット

HBB

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Nature communications 11:6316 PubMed33298911

2020

TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Feng Wang,Zhao-Na Yang,Ting-Ting Zhang,Yu-Fen Yuan,Chen-Xi Zhao,Zaiwuli Yeerjiang,Bing Cui,Fang Hua,Xiao-Xi Lv,Xiao-Wei Zhang,Jiao-Jiao Yu,Shan-Shan Liu,Jin-Mei Yu,Shuang Shang,Yang Xiao,Zhuo-Wei Hu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hemoglobin subunit beta + Hemoglobin subunit delta 抗体 [EPR8322(B)]

Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (AB131225)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (2)

Abcam product promise