Anti-Hemoglobin 抗体 (ab19363)
Key features and details
- Sheep polyclonal to Hemoglobin
- Suitable for: Immunoelectrophoresis, Double Immunodiffusion
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hemoglobin antibody
Hemoglobin 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to Hemoglobin -
由来種
Sheep -
特異性
By immunoelectrophoresis, the antiserum reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins
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アプリケーション
適用あり: Immunoelectrophoresis, Double Immunodiffusionmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human Hemoglobin. Sheep were immunized with purified human hemoglobin F of fetal red blood cell origin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 1.21% Tris, 0.02% Sodium chloride -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Antiserum was solid phase adsorbed against hemoglobin A to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19363の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Immunoelectrophoresis |
Use at an assay dependent concentration.
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Double Immunodiffusion |
Use at an assay dependent concentration.
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特記事項 |
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Immunoelectrophoresis
Use at an assay dependent concentration. |
Double Immunodiffusion
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in oxygen transport from the lung to the various peripheral tissues. -
組織特異性
Red blood cells. -
関連疾患
Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
配列類似性
Belongs to the globin family. -
翻訳後修飾
The initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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参照データベース
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Entrez Gene: 3043 Human
- Entrez Gene: 3047 Human
- Omim: 141800 Human
- Omim: 141900 Human
- Omim: 142200 Human
- SwissProt: P68871 Human
see all -
別名
- 3-prime alpha-globin gene antibody
- A gamma globin antibody
- Alpha 1 globin antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab19363 は論文での使用が確認できていません。