Name: Anti-Heme Oxygenase 1 antibody [HO-1-1]
SKU: ab13248
Rating: 4 (19 reviews)

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Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Key features and details

Mouse monoclonal [HO-1-1] to Heme Oxygenase 1
Suitable for: WB, Flow Cyt (Intra), Sandwich ELISA, IHC-P
Reacts with: Mouse, Rat, Cow, Dog, Human
Isotype: IgG1

製品名

Anti-Heme Oxygenase 1 antibody [HO-1-1]
Heme Oxygenase 1 一次抗体製品一覧
製品の詳細

Mouse monoclonal [HO-1-1] to Heme Oxygenase 1
由来種

Mouse
アプリケーション

適用あり: WB, Flow Cyt (Intra), Sandwich ELISA, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Cow, Dog, Human
交差が予測される動物種: Pig
免疫原

Synthetic peptide:
MERPQPDSMPQDLSEALKEATKEVHTQAEN
, corresponding to amino acids 1-30 of Human Heme Oxygenase 1.
Run BLAST with Run BLAST with
ポジティブ･コントロール
- Recombinant Human or Rat HO-1 (Hsp32) Protein. HEK293 treated with 30 uM hemin for 18hrs (see Abreview). IHC-P: FFPE human spleen normal.
特記事項

This product was changed from ascites to tissue culture supernatant on 22^nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
精製度

Tissue culture supernatant
特記事項（精製）

Purified from TCS.
ポリ/モノ

モノクローナル
クローン名

HO-1-1
アイソタイプ

IgG1
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Recombinant Protein
- Recombinant Human Heme Oxygenase 1 protein (ab85243)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
- Recombinant Human Heme Oxygenase 1 protein (ab85243)
sELISA pair antibody
- Anti-Heme Oxygenase 1 antibody (ab13243)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab13248の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(15)	Use a concentration of 4 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
Flow Cyt (Intra)		Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Sandwich ELISA		Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to Heme Oxygenase 1 (ab13243). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Heme Oxygenase 1 (ab13243) as Detection.
IHC-P	(1)	Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

特記事項
WB Use a concentration of 4 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
Flow Cyt (Intra) Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to Heme Oxygenase 1 (ab13243). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Heme Oxygenase 1 (ab13243) as Detection.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

機能

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
配列類似性

Belongs to the heme oxygenase family.
細胞内局在

Microsome. Endoplasmic reticulum.
Target information above from: UniProt accession P09601 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 513221 Cow
- Entrez Gene: 442987 Dog
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 445512 Pig
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: Q5E9F2 Cow
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P32394 Pig
- SwissProt: P06762 Rat
- Unigene: 517581 Human
- Unigene: 276389 Mouse
- Unigene: 3160 Rat
see all
別名
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
- heat shock protein 32 kD antibody
- heat shock protein 32kD antibody
- Heat shock protein antibody
- Heme oxygenase (decycling) 1 antibody
- Heme oxygenase 1 antibody
- Hemox antibody
- HMOX 1 antibody
- Hmox antibody
- Hmox1 antibody
- HMOX1_HUMAN antibody
- HO 1 antibody
- HO antibody
- HO-1 antibody
- HO1 antibody
- Hsp32 antibody
see all

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

All lanes : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/ml

Lane 1 : Hek293
Lane 2 : HL60
Lane 3 : HeLa
Lane 4 : A549
Lane 5 : Hu spleen
Lane 6 : Ms spleen
Lane 7 : Rt spleen

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : IRDye® 800CW Goat anti Mouse

Predicted band size: 34.6 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Hek293 & HL60 presumed negative or very low expression.

Loading control GAPDH at 38kDa

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

This image was generated using the ascites version of the product.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

The following proteins and lysates were electrophoresed; Lane 1 - Heme-Oxygenase-1 (Hsp32) Protein (50ng), lane 2 - Heme-Oxygenase-2 protein NSP-550 (100ng), lane 3 - MDBK Cell Lysate (20ug), lane 4 - Mouse liver microsome (20ug) and lane 5 - Dog liver microsome (20ug). ab13248 was applied at a concentration of 4ugml-1.

This image was generated using the ascites version of the product.
Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.

This image was generated using the ascites version of the product.
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Lane 1 : MW marker
Lanes 2-7 : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Lane 2 : Recombinant Rat Heme Oxygenase 1
Lane 3 : Recombinant Human Heme Oxygenase 1
Lane 4 : Recombinant Human Heme Oxygenase 2
Lane 5 : MDBK cell lysate
Lane 6 : Dog liver microsome
Lane 7 : Mouse liver microsome

Predicted band size: 34.6 kDa

This image was generated using the ascites version of the product.
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution + Human microsome lysate

Predicted band size: 34.6 kDa

This image was generated using the ascites version of the product.
Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

ab13248 at 10µg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.

This image was generated using the ascites version of the product.

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols
Protocol Booklet

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab13248 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13248 は 329 報の論文で使用されています。

Song Z et al. Hyperphosphorylated Tau Inflicts Intracellular Stress Responses that Are Mitigated by Apomorphine. Mol Neurobiol 61:2653-2671 (2024). PubMed: 37919601
Carta G et al. Transcriptional landscape of mitochondrial electron transport chain inhibition in renal cells. Cell Biol Toxicol 39:3031-3059 (2023). PubMed: 37353587
Hu LT et al. Exosomal miR-23b from bone marrow mesenchymal stem cells alleviates oxidative stress and pyroptosis after intracerebral hemorrhage. Neural Regen Res 18:560-567 (2023). PubMed: 36018178
Wu J et al. Gambogenic acid induces apoptosis and autophagy through ROS-mediated endoplasmic reticulum stress via JNK pathway in prostate cancer cells. Phytother Res 37:310-328 (2023). PubMed: 36086867
Ma J et al. GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment. Adv Healthc Mater 12:e2202377 (2023). PubMed: 36549669

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 44 Abreviews or Q&A

Application

Western blot

Sample

Human Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (10% polyacrilamide gel Wet transfer at 400mA for 90 min)

Loading amount

12.5 µg

Specification

Skeletal muscle

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 07 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mC166 cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Treatment

100uM H2O2

Specification

mC166 cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yue Zhao

Verified customer

投稿 Jul 09 2021

Application

Western blot

Sample

Rat Cell lysate - whole cell (PC12 cell)

Gel Running Conditions

Non-reduced Denaturing (12%)

Loading amount

20 µg

Specification

PC12 cell

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Ji Yeon Lee

Verified customer

投稿 Jan 25 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (DU145)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

25 µg

Treatment

20nM siNeg or siNFE2L2 for 24, 48 and 72h

Specification

DU145

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 17 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate

Permeabilization

Specification

Spleen

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 23 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 14 2016

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (8%)

Sample

Rat Cell lysate - whole cell (RAT AORTIC SMOOTH MUSCLE CELLS)

Specification

RAT AORTIC SMOOTH MUSCLE CELLS

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. XXXXXX XXXXXX

Verified customer

投稿 Oct 14 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioblastoma primary cells)

Loading amount

30 µg

Specification

Glioblastoma primary cells

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 16 2013

Question

1) Abcam product code ab 13248
2) Abcam order reference number or product batch number
3) Description of the problem : unable to detect bands in western blot
4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein…): cell lysate
Lysis buffer 1x lysis buffer consisting of tris and SDS
Protease inhibitors: none
Phosphatase inhibitors none
Reducing agent none
Boiling for ≥5 min? yes/no
Protein loaded ug/lane or cells/lane 10-15ug perlane
Positive control rat spleen
Negative control none
5) Percentage of gel 8%
Type of membrane PVDF
Protein transfer verified
Blocking agent and concentration non-fat dry milk (5%), wash buffer, tween20 and distilled water
Blocking time atleast 1hr
Blocking temperature room temp.
6) Primary antibody (If more than one was used, describe in “additional notes”) : anti HO-1
Concentration or dilution 4ug/ml
Diluent buffer blocking buffer
Incubation time overnight
Incubation temperature: 40c
7) Secondary antibody: anti-mouse
Species:
Reacts against:
Concentration or dilution 1:5000
Diluent buffer blocking buffer
Incubation time 1hour
Incubation temperature: room temp.
Fluorochrome or enzyme conjugate: HRP linked
8) Washing after primary and secondary antibodies:
Buffer wash buffer (1x) with 0.1% tween20
Number of washes 3 times after primary and 5 times after secondary
9)Detection method
10) How many times have you run this staining? 3 times
Do you obtain the same results every time? yes
What steps have you altered to try and optimize the use of this antibody? none
Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.

Abcam community

Verified customer

Asked on Oct 25 2012

Answer

Thank you for contacting us.

I have checked the details you have kindly provided. I would suggest the following troubleshooting steps;

- Please add cocktail of protease inhibitors in lysates.
- This antibody works perfect in reducing conditions. Please add DTT in sample buffer.
- Try heating the lysates at 100C for 10 minutes.
- Load 20-30ug of lysates.
- Try 5-10 ug/ml of antibody concentration.
- Please make sure the secondary is a anti-mouse antibody.

Please try these steps and let me know the results.

Many thanks for your cooperation. I will look forward to hearing from you soon.

Abcam Scientific Support

Answered on Oct 25 2012

Question

- Antiboy does not work with Rat spleen lysates
- Lysates used 10ug

Abcam community

Verified customer

Asked on Oct 23 2012

Answer

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Abcam Scientific Support

Answered on Oct 23 2012

1-10 of 44 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

sELISA pair antibody

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (329)

レビューと Q&A

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Immunohistochemistry (Frozen sections) abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]

Western blot abreview for Anti-Heme Oxygenase 1 antibody [HO-1-1]