Anti-Heme Oxygenase 1 抗体 (ab85309)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Heme Oxygenase 1 antibody
Heme Oxygenase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Heme Oxygenase 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Heme Oxygenase 1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94374) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| IHC-P | (3) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
配列類似性
Belongs to the heme oxygenase family. -
細胞内局在
Microsome. Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P06762 Rat
- Unigene: 517581 Human
see all -
別名
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
画像
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Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : HEK-293
Lane 2 : HL60
Lane 3 : Rt spleen
Lane 4 : NR8383
Lane 5 : Ms spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C) on human lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins at 21°C. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: Positivity appears to be confined to macrophages- the primary antibody was omitted on the sequential section and was negative for macrophages ( to rule out lipofuscin).
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Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 47 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C)on rat lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: As well as in macrophage cytoplasm, there is also clear positivity in the lining epitheium of the respiratory conducting tubes. I do not know if this is specific/non-specific.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab85309 は 5 報の論文で使用されています。
- Huang X et al. Transcriptome Analysis of Protection by Dendrobium Nobile Alkaloids (DNLA) against Chronic Alcoholic Liver Injury in Mice. Biomedicines 10:N/A (2022). PubMed: 36359319
- Sohn E et al. Ficus erecta Thunb Leaves Alleviate Memory Loss Induced by Scopolamine in Mice via Regulation of Oxidative Stress and Cholinergic System. Mol Neurobiol 58:3665-3676 (2021). PubMed: 33797061
- Li YY et al. Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion. Kaohsiung J Med Sci 35:417-424 (2019). PubMed: 30977589
- Chen X et al. Ethanol extract of Schisandrae chinensis fructus ameliorates the extent of experimentally induced atherosclerosis in rats by increasing antioxidant capacity and improving endothelial dysfunction. Pharm Biol 56:612-619 (2018). PubMed: 31070526
- Kataoka K et al. Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice. Int J Mol Sci 17:N/A (2016). IHC-P ; Mouse . PubMed: 27854327