Anti-Heme Oxygenase 1 抗体 (ab13243)

Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nun damit verarbeitet bekommen kann ich leider nicht versprechen, da die Antikörper ja auch in den meisten Fällen ein paar Optimierungsexperimente benötigen.

https://www.abcam.com/TNF-alpha-antibody-ab9739.html (or use the following: https://www.abcam.com/TNF-alpha-antibody-ab9739.html).

https://www.abcam.com/IL-10-antibody-ab9969.html (or use the following: https://www.abcam.com/IL-10-antibody-ab9969.html).

https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html (or use the following: https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html).

https://www.abcam.com/IL-6-antibody-ab83339.html (or use the following: https://www.abcam.com/IL-6-antibody-ab83339.html).


Als Sekundär-AK würde ich Ihnen den ab97069 Goat anti-Rabbit IgG H&L (HRP) secondary antibody empfehlen, da dieser bis zu einer Verdünnung von 1/50.000 eingestzt werden kann.



https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html).


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere technische Fragen haben. Falls Sie die Produkte Bestellen möchten oder mehr Informationen zB bezüglich des Preises wünschen, wenden Sie sich einfach an unseren Kundendienst.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Thank you for your reply.

I will then let the order through as it stands. We will endeavor to have the product with you as soon as possible but as I am sure you can understand we are reliant on delivery companies to move the package on our behalf. I will keep you updated with its progress.

As for the BCA kit and the extra BSA you have received compared to the 1 mL described. This is not unusual, we tend to supply a larger volume of all our products than we advertise to take into account any pipetting performed on our side. We want to make sure that the customer never receives less than expected and therefore typically over fill, with up to 10% of product. This should have no effect on the concentration of BSA supplied.

We do advise aliquotting the Anti-Heme Oxygenase 1 antibody when stored at -20°C regardless of the fact that it is in 50% glycerol. This is to avoid the antibody going through multiple freeze/thaw cycles (or more accurately with the glycerol added, temperature changes). This can affect the activity of the antibody and as a precautionary measure this is what we would advise. It also helps in that it can reduce the chance of contamination of the stock.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

Thank you for your reply.

We are indeed currently running an offer with our Cell Imaging book if you purchase any secondary antibody. Unfortunately I am unable to extend this offer to primary antibodies. I have had a look at the book and it is a very valuable resourcefor live cell imaging and light microscopy application. A review of the content can be found from the following link:

http://www.scionpublishing.com/shop/ProductImages/Cell%20Imaging%20Book%20Review.pdf

I think it is well worth getting if you are interested in this type of imaging. If your interested in the offer, please just quote the code CELL-TBVCQ with any secondary antibody order. More information on the offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=14705

In regards to a positive control tissue, we have found the protein to be highly expressed in liver and spleen tissue and so if you have access to this it may prove a useful control. Otherwise I would suggest having a look through the journals to see what other researchers have used in their experiments in conjunction to staining of Heme Oxygenase 1.

I hope this information has been of some help. If you have any further questions, please do not hesitate to contact us again.

I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:

HO-1

I would suggest looking at the rabbit polyclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html. This has been shown to react with human, mouse and rat samples and has been used in ICC, IHC-Paraffin embedded, sELISA and Western blotting to date. We also have a very popular mouse monoclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-HO-1-1-ab13248.htmlwhich has also been used with human, mouse and rat samples as well as in ELISA, Flow Cytometry, ICC,IHC-Frozen sections, and Western blotting. Bit I think you were more interested in antibodies raised in rabbit.

COX-2

For this target I would suggest having a look at the rabbit polyclonal https://www.abcam.com/COX2--Cyclooxygenase-2-antibody-ab15191.html. This has again been tested with mouse, rat and human samples and has been used in ICC/IF, IHC-Frozen sections, IHC-Parrafin embedded, IP, sELISA and Westernblotting.

We also have the NS-398 compound (COX-2 inhibitor) which is available under the catalogue number https://www.abcam.com/NS-398-ab120295.html.

I am afraid I have not been able to find a similar inhibitor of VEGF. Do you have a particular compound in mind? It may be that I can suggest we start stocking it.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Thank you very much for your reply.

We are sending a free of charge vial of ab79854 on the order ***, which should arrive tomorrow. Please keep me updated about the results with this replacement, and let me know if there is anything else that we can do for you.

Have a nice day.