Anti-HEC1/HEC 抗体 (ab186839)
Key features and details
- Rabbit polyclonal to HEC1/HEC
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HEC1/HEC antibody
HEC1/HEC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HEC1/HEC -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human HEC1/HEC.
Database link: O14777 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186839の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 74 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 74 kDa. |
ターゲット情報
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機能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
配列類似性
Belongs to the NDC80/HEC1 family. -
発生段階
Expression peaks in mitosis. -
翻訳後修飾
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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参照データベース
- Entrez Gene: 10403 Human
- Entrez Gene: 67052 Mouse
- Omim: 607272 Human
- SwissProt: O14777 Human
- SwissProt: Q9D0F1 Mouse
- Unigene: 414407 Human
- Unigene: 225956 Mouse
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別名
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
see all
画像
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Western blot - Anti-HEC1/HEC antibody (ab186839)All lanes : Anti-HEC1/HEC antibody (ab186839) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : LOVO cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : 22RV-1 cell lysate
Lane 5 : 293T cell lysate
Lane 6 : mouse testis lysate
Lane 7 : THP1 cell lysate
Predicted band size: 74 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186839 は 1 報の論文で使用されています。
- Li Y et al. Histone Deacetylase Inhibitor Trichostatin A Reduces Endothelial Cell Proliferation by Suppressing STAT5A-Related Gene Transcription. Front Oncol 11:746266 (2021). PubMed: 34650929