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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-HDAC8 抗体 [EPR10338(2)] (ab187139)

Reviews (1) Submit a question References (33)
Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10338(2)] to HDAC8
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HDAC8 antibody [EPR10338(2)]
    HDAC8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10338(2)] to HDAC8

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IPmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, Molt4 and K562 cell lysates; human kidney tissue lysate; K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187139の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IP
1/40 - 1/60.
特記事項
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IP
1/40 - 1/60.

ターゲット情報

  • 機能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.

  • 組織特異性

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

  • 配列類似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • 翻訳後修飾

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

  • 細胞内局在

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

画像

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HADC8 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
    ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    All lanes : Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Molt4 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.

  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG. 

     

     

  • Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

参考文献 (33)

ab187139 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187139 は 33 報の論文で使用されています。

  • Kim JY  et al. HDAC8 Deacetylates HIF-1α and Enhances Its Protein Stability to Promote Tumor Growth and Migration in Melanoma. Cancers (Basel) 15:N/A (2023). PubMed: 36831463
  • Chervy M  et al. Epigenetic master regulators HDAC1 and HDAC5 control pathobiont Enterobacteria colonization in ileal mucosa of Crohn's disease patients. Gut Microbes 14:2127444 (2022). PubMed: 36175163
  • Zhao T  et al. Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1. Oxid Med Cell Longev 2022:6227330 (2022). PubMed: 35126818
  • Lu Z  et al. Promotion of microRNA-146a by histone deacetylase 4 silencing contributes to radiosensitization of esophageal carcinoma. J Transl Med 20:101 (2022). PubMed: 35193602
  • Kusakabe M  et al. Histone deacetylation regulates nucleotide excision repair through an interaction with the XPC protein. iScience 25:104040 (2022). PubMed: 35330687
View all Publications for this product

レビューと Q&A

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Western blot abreview for Anti-HDAC8 antibody [EPR10338(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
12 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Apr 02 2016

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