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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-HDAC6 抗体 [EPR1698(2)] (ab133493)

Reviews (1) Submit a question References (14)
Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
  • Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
  • Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
  • Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
  • Anti-HDAC6 antibody [EPR1698(2)] (ab133493)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1698(2)] to HDAC6
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ChIC/CUT&RUN-seq
  • Knockout validated
  • Reacts with: Human, African green monkey

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-HDAC6 antibody [EPR1698(2)]
    HDAC6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1698(2)] to HDAC6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ChIC/CUT&RUN-seqmore details

  • 種交差性

    交差種: Human, African green monkey
    交差が予測される動物種: Monkey

  • 免疫原

    Synthetic peptide within Human HDAC6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBN7

  • ポジティブ･コントロール

    • WB: HAP1, HeLa, Jurkat, and COS-1 cell lysates. Flow Cyt (intra): K562. IHC-P: Human kidney tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab133493の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200.
WB (1)
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
1/200.
WB
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

  • 配列類似性

    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.

  • 翻訳後修飾

    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.

  • 細胞内局在

    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CPBHM antibody
      • FLJ16239 antibody
      • HD 6 antibody
      • HD6 antibody
      • HDAC 6 antibody
      • HDAC6 antibody
      • HDAC6_HUMAN antibody
      • Histone deacetylase 6 (HD6) antibody
      • Histone deacetylase 6 antibody
      • JM 21 antibody
      • JM21 antibody
      • KIAA0901 antibody
      • OTTHUMP00000032398 antibody
      • OTTHUMP00000197663 antibody
      • PPP1R90 antibody
      • Protein phosphatase 1 regulatory subunit 90 antibody
      see all

    画像

    • Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : HDAC6 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab133493 was shown to react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line was used. Wild-type HeLa and HDAC6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)

      ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HDAC6 with ab133493 antibody at a dilution of 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Lanes 1-2 : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
      Lanes 3-4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution


      Lanes 1 & 3 & 5 : Wild-type HAP1 cell lysate
      Lanes 2 & 4 & 6 : HDAC6 knockout HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 131 kDa



      Lanes 1 and 2: Green signal from target – ab133493 observed at 160 kDa
      Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
      Lanes 5 and 6: Merged (red and green) signal

      ab133493 was shown to specifically react with HDAC6 when HDAC6 knockout samples were used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. ab133493 and ab8226 (loading control to beta actin) were diluted 1/10 000 and 1/1000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Western blot - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : COS-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 131 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?

    • Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
      Anti-HDAC6 antibody [EPR1698(2)] (ab133493)

    参考文献 (14)

    ab133493 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab133493 は 14 報の論文で使用されています。

    • Yang D  et al. Pharmacological Targeting of Bcl-2 Induces Caspase 3-Mediated Cleavage of HDAC6 and Regulates the Autophagy Process in Colorectal Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 37047634
    • Zhao T  et al. Qi-Dong-Huo-Xue-Yin balances the immune microenvironment to protect against LPS induced acute lung injury. Front Pharmacol 14:1200058 (2023). PubMed: 37292149
    • Nie Q  et al. Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel. Mol Med Rep 28:N/A (2023). PubMed: 37681444
    • Shin HS  et al. Histone Deacetylase as a Valuable Predictive Biomarker and Therapeutic Target in Immunotherapy for Non-Small Cell Lung Cancer. Cancer Res Treat 54:458-468 (2022). PubMed: 34517693
    • Yuan B  et al. Patient-derived organoids for personalized gallbladder cancer modelling and drug screening. Clin Transl Med 12:e678 (2022). PubMed: 35075805
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-HDAC6 antibody [EPR1698(2)]

     Average
    Abreviews
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing (7,5)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Oct 23 2014

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