Anti-HDAC6 抗体 (ab117516)
Key features and details
- Rabbit polyclonal to HDAC6
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HDAC6 antibody
HDAC6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC6 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human HDAC6 aa 7-56 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9UBN7 -
ポジティブ・コントロール
- Human intestine (ganglion cells) and Human testis tissues; HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+, Ca2+ -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117516の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 131 kDa.
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IHC-P |
1/200.
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ICC/IF | (4) |
1/100 - 1/500.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 131 kDa. |
IHC-P
1/200. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
翻訳後修飾
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
細胞内局在
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
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別名
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
画像
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All lanes : Anti-HDAC6 antibody (ab117516) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with immunizing peptide
Predicted band size: 131 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody (ab117516)
ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody (ab117516)
ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.
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ab117516, at 1/100, staining HDAC6 in HepG2 cells by Immunofluorescence (Left image).
The image on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab117516 は 4 報の論文で使用されています。
- Babu G et al. JAK-STAT inhibitor as a potential therapeutic opportunity in AML patients resistant to cytarabine and epigenetic therapy. Cancer Biol Ther 22:66-78 (2021). PubMed: 33356802
- Manca S et al. The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol Cancer Res 17:225-237 (2019). PubMed: 30224543
- Fan T et al. Autophagy Activation by Rapamycin Before Hypoxia-Reoxygenation Reduces Endoplasmic Reticulum Stress in Alveolar Epithelial Cells. Cell Physiol Biochem 41:79-90 (2017). PubMed: 28214844
- Hanigan TW et al. Divergent JNK Phosphorylation of HDAC3 in Triple-Negative Breast Cancer Cells Determines HDAC Inhibitor Binding and Selectivity. Cell Chem Biol 24:1356-1367.e8 (2017). PubMed: 28943357