Anti-HDAC5 (phospho S498) 抗体 (ab47283)
Key features and details
- Rabbit polyclonal to HDAC5 (phospho S498)
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HDAC5 (phospho S498) antibody
HDAC5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC5 (phospho S498) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
Database link: Q9UQL6 -
ポジティブ･コントロール
- NIH/3T3 cell extracts; Breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47283の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
ドメイン
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻訳後修飾
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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参照データベース
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Entrez Gene: 84580 Rat
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
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別名
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
画像
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Western blot - Anti-HDAC5 (phospho S498) antibody (ab47283)All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution
Lane 1 : Extracts from NIH/ 3T3 cells
Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)
Predicted band size: 122 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 (phospho S498) antibody (ab47283)ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab47283 は 14 報の論文で使用されています。
- Truong V et al. Angiotensin II-induced histone deacetylase 5 phosphorylation, nuclear export, and Egr-1 expression are mediated by Akt pathway in A10 vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 320:H1543-H1554 (2021). PubMed: 33606583
- Pietruczuk P et al. Protein kinase B mediates insulin-like growth factor 1-induced phosphorylation and nuclear export of histone deacetylase 5 via NADPH oxidase 4 activation in vascular smooth muscle cells. J Cell Physiol N/A:N/A (2019). PubMed: 30793765
- Tóth AD et al. Inflammation leads through PGE/EP3 signaling to HDAC5/MEF2-dependent transcription in cardiac myocytes. EMBO Mol Med 10:N/A (2018). PubMed: 29907596
- Meng ZX et al. Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis. Mol Cell 66:332-344.e4 (2017). PubMed: 28475869
- Weeks KL et al. ß-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55a-PP2A-Mediated Dephosphorylation. J Am Heart Assoc 6:N/A (2017). PubMed: 28343149