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AB39408

Anti-HDAC4 (phospho S632) 抗体

Anti-HDAC4 (phospho S632) antibody

4

(3 Reviews)

|

(14 Publications)

Rabbit Polyclonal HDAC4 phospho S632 antibody. Suitable for ELISA, WB and reacts with Mouse, Rat, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC4 phospho S632 aa 550-650.

別名を表示する

KIAA0288, HDAC4, Histone deacetylase 4, HD4

3 Images
Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)
  • WB

Collaborator25143****

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

Lane 1:

Lysate from murine heart tissue 1 at 20 µg

Lane 2:

Lysate from murine heart tissue 2 at 20 µg

Secondary

All lanes:

HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution

Predicted band size: 119 kDa

Observed band size: 130 kDa

true

Exposure time: 1min

Image courtesy of an anonymous Abreview.

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)
  • WB

PubMed

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit.

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)

Predicted band size: 119 kDa

false

Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)
  • WB

Unknown

Western blot - Anti-HDAC4 (phospho S632) antibody (AB39408)

All lanes:

Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

Lane 1:

Extract from Jurkat cells (5-30ug of total protein)

Lane 2:

Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)

Predicted band size: 119 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human, Rat

アプリケーション

ELISA, WB

applications

免疫原

Synthetic Peptide within Human HDAC4 phospho S632 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB279820

Phospho-HDAC4 (S632) ELISA Kit

0

0 Reviews

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こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4 phospho S632
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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 32:704-720 PubMed37234747

2023

Silencing of microRNA-106b-5p prevents doxorubicin-mediated cardiotoxicity through modulation of the PR55α/YY1/sST2 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Lax,Fernando Soler,Maria Josefa Fernandez Del Palacio,Silvia Pascual-Oliver,Miriam Ruiz Ballester,Jose Javier Fuster,Domingo Pascual-Figal,Maria Del Carmen Asensio-Lopez

Journal of cellular physiology 236:7001-7013 PubMed33724469

2021

PTHrP promotes development of mouse preimplantation embryos through the AKT/cyclin D1 pathway and nuclear translocation of HDAC4.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Li,Lei Guo,Hui Li,Wen-Long Lei,Li-Hua Fan,Ying-Chun Ouyang,Yi Hou,Zhen-Bo Wang,Qing-Yuan Sun,Sheng-Sheng Lu,Zhiming Han

Redox biology 36:101669 PubMed32818796

2020

Oxidation of HDAC4 by Nox4-derived HO maintains tube formation by endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Schader,Oliver Löwe,Christina Reschke,Pedro Malacarne,Fabian Hahner,Niklas Müller,Anna Gajos-Draus,Johannes Backs,Katrin Schröder

Journal of cachexia, sarcopenia and muscle 11:89-102 PubMed31743617

2019

Effects of aerobic and inspiratory training on skeletal muscle microRNA-1 and downstream-associated pathways in patients with heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Ligia M Antunes-Correa,Patricia F Trevizan,Aline V N Bacurau,Larissa Ferreira-Santos,João L P Gomes,Ursula Urias,Patricia A Oliveira,Maria Janieire N N Alves,Dirceu R de Almeida,Patricia C Brum,Edilamar M Oliveira,Ludhmila Hajjar,Roberto Kalil Filho,Carlos Eduardo Negrão

Journal of molecular and cellular cardiology 130:216-233 PubMed30998979

2019

Yin-Yang 1 transcription factor modulates ST2 expression during adverse cardiac remodeling post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

M C Asensio-Lopez,A Lax,M J Fernandez Del Palacio,Y Sassi,R J Hajjar,J L Januzzi,A Bayes-Genis,D A Pascual-Figal

Biochimica et biophysica acta. Gene regulatory mec 1862:493-508 PubMed30831269

2019

Leukocyte integrin signaling regulates FOXP1 gene expression via FOXP1-IT1 long non-coding RNA-mediated IRAK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Can Shi,Jessica Miley,Alison Nottingham,Toshifumi Morooka,Domenick A Prosdocimo,Daniel I Simon

American journal of physiology. Heart and circulat 315:H1463-H1476 PubMed30141986

2018

Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Estibaliz Castillero,Ziad A Ali,Hirokazu Akashi,Nicholas Giangreco,Catherine Wang,Eric J Stöhr,Ruping Ji,Xiaokan Zhang,Nathaniel Kheysin,Joo-Eun S Park,Sheetal Hegde,Sanatkumar Patel,Samantha Stein,Carlos Cuenca,Diana Leung,Shunichi Homma,Nicholas P Tatonetti,Veli K Topkara,Koji Takeda,Paolo C Colombo,Yoshifumi Naka,H Lee Sweeney,P Christian Schulze,Isaac George

Oncology letters 15:3252-3258 PubMed29435066

2018

Potential anticancer effect of prostratin through SIK3 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Dalal Alotaibi,Suneetha Amara,Terrance L Johnson,Venkataswarup Tiriveedhi

PloS one 12:e0180097 PubMed28658303

2017

Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Suneetha Amara,Ciera Majors,Bipradas Roy,Salisha Hill,Kristie L Rose,Elbert L Myles,Venkataswarup Tiriveedhi

Cardiovascular research 111:287-94 PubMed27131508

2016

Elevated local [Ca2+] and CaMKII promote spontaneous Ca2+ release in ankyrin-B-deficient hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Iuliana Popescu,Samuel Galice,Peter J Mohler,Sanda Despa
View all publications
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Abcam product promise

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