Anti-HDAC4 抗体 (ab12172)
Key features and details
- Rabbit polyclonal to HDAC4
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HDAC4 antibody
HDAC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Chicken
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免疫原
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. -
ポジティブ･コントロール
- WB: NIH/3T3 whole cell extract, Rat brain cell extract. IP: HeLa whole cell extract. ICC/IF: HeLa tissue.
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特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before useThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/250 - 1/500.
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| WB | (5) |
1/500 - 1/2000. Predicted molecular weight: 119 kDa.
Additional weak bands may be detected when blotting using different extract preparations. |
| IP |
Use at an assay dependent concentration.
10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
| 特記事項 |
|---|
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ICC/IF
1/250 - 1/500. |
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WB
1/500 - 1/2000. Predicted molecular weight: 119 kDa. Additional weak bands may be detected when blotting using different extract preparations. |
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IP
Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. -
組織特異性
Ubiquitous. -
関連疾患
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
ドメイン
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻訳後修飾
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
別名
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
画像
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Western blot - Anti-HDAC4 antibody (ab12172)Lane 1 : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 2 : Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lanes 1-2 : Rat brain whole extract
Lane 3 : Negative control
Predicted band size: 119 kDa -
Western blot - Anti-HDAC4 antibody (ab12172)Lane 1 : Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lane 2 : Anti-HDAC4 antibody (ab12172) at 1/2000 dilution
Lanes 1-2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract
Lane 3 : Negative Control
Predicted band size: 119 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (ab12172)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution.
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Immunoprecipitation - Anti-HDAC4 antibody (ab12172)Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.Lane 1: 10 µg of ab12172Lane 2: 5 µg of ab12172Negative control: No cell extractPositive control: HeLa whole cell extract
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab12172 は 33 報の論文で使用されています。
- Colussi C et al. Cytoplasmic HDAC4 recovers synaptic function in the 3×Tg mouse model of Alzheimer's disease. Neuropathol Appl Neurobiol 49:e12861 (2023). PubMed: 36331820
- Xu Y et al. HDAC4 regulates the proliferation, migration, and invasion of trophoblasts in pre-eclampsia through the miR-134-5p/FOXM1 axis. Mol Reprod Dev 90:849-860 (2023). PubMed: 37769062
- Lv Z et al. Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide. JCI Insight 8:N/A (2023). PubMed: 37870958
- Ha N et al. Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development. Front Physiol 13:819619 (2022). PubMed: 35242053
- Wedge ME et al. Virally programmed extracellular vesicles sensitize cancer cells to oncolytic virus and small molecule therapy. Nat Commun 13:1898 (2022). PubMed: 35393414