AB32369

Anti-HDAC3 抗体 [Y415]

Name: Anti-HDAC3 抗体 [Y415] (ab32369) | Abcam
Brand: Abcam Limited
SKU: AB32369
Price: 33000 JPY
Availability: InStock
Rating: 5 (6 reviews)

Anti-HDAC3 antibody [Y415]

RabMAb
Recombinant
20ul selling size
詳細を見る

(6 Reviews)

(92 Publications)

Anti-HDAC3 antibody [Y415] (ab32369) is a rabbit monoclonal antibody detecting HDAC3 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications
- Trusted since 2006

別名を表示する

Histone deacetylase 3, HD3, Protein deacetylase HDAC3, Protein deacylase HDAC3, RPD3-2, SMAP45, HDAC3

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369)

Immunofluorescent staining of HeLa cells using ab32369 at 1/250 dilution

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369)

Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling HDAC3 with purified ab32369 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor^®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used and for negative control 2, mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (AB32369)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (AB32369)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HDAC3 with purified ab32369 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369)

Paraffin-embedded human ovary carcinoma
ab32369 at 1/250 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369)

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma sections labelling HDAC3 with purified ab32369 at dilution of 1 : 50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Lab

Immunoprecipitation - Anti-HDAC3 antibody [Y415] (AB32369)

Purified ab32369 at 1/20 immunoprecipitating HDAC3 in K562 whole cell lysate observed at 49 KDa (lanes 1 and 2).

Lane 1 (input) : K562 whole cell lysate 10ug

Lane 2 (+) : ab32369 + K562 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32369 in K562 whole cell lysate

The secondary antibody used was VeriBlot for IP Detection Reagent (HRP) (ab131366) for detection at dilution of 1/1000.

Blocking/Diluting buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369)

Predicted band size: 48 kDa

false

Unknown

Western blot - Anti-HDAC3 antibody [Y415] (AB32369)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-HDAC3 antibody [Y415] (ab32369) at 1/5000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

Lane 4:

C6 (rat glioma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Unknown

Western blot - Anti-HDAC3 antibody [Y415] (AB32369)

All lanes:

Western blot - Anti-HDAC3 antibody [Y415] (ab32369) at 1/10000 dilution

All lanes:

K562 cell lysate

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

Y415

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

Flow Cyt (Intra), ICC/IF, IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody may detect splice isoform 2 (RPD3-2A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-HDAC3 antibody [Y415] (ab32369) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of HDAC3?
Anti-HDAC3 [Y415] (ab32369) specifically detects a band for HDAC3 (UniProt: O15379) at a molecular weight of 49kDa.

Trusted by the scientific community
Anti-HDAC3 [Y415] (ab32369) was first used in a scientific publication in 2006 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-HDAC3 [Y415] (ab32369) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [Y415] also available for your convenience: ab32369, HRP - ab208356, Carrier free - ab219376, APC - ab310854, PE - ab310923, Alexa Fluor® 488 - ab310969, Alexa Fluor® 647 - ab311086, Alexa Fluor® 594 - ab311683, Alexa Fluor® 568 - ab312958, Alexa Fluor® 555 - ab313167, Alexa Fluor® 750 - ab321594

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.12% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HDAC3 or Histone Deacetylase 3 belongs to the class I histone deacetylases and plays an important mechanical role in epigenetic regulation by removing acetyl groups from histone proteins which causes chromatin condensation and gene expression modulation. Often referred to by its alternate name 3g6 HDAC3 has a molecular weight of approximately 49 kDa. The protein expresses broadly in various tissues including the brain liver and heart highlighting its widespread regulatory function across different body systems.

Biological function summary

HDAC3 acts as part of the nuclear receptor corepressor complex engaging with other subunits to modify chromatin structure and gene transcription. This protein influences various cellular processes such as cell cycle regulation and apoptosis. By altering chromatin architecture HDAC3 modulates the expression of numerous genes supporting cellular homeostasis and response to environmental signals.

Pathways

The HDAC3 protein integrates into key signaling networks including the Notch and NF-kB pathways. Within the Notch signaling pathway HDAC3 interacts with proteins like CSL modulating transcriptional responses critical for cell differentiation and developmental processes. In the NF-kB pathway it influences inflammation and immune responses by regulating the transcription of inflammatory genes showcasing its role in mediating complex signaling cascades.

HDAC3 is pivotal in the development and progression of various diseases such as cancer and neurodegenerative disorders. The dysregulation of HDAC3 activity can lead to abnormal cell proliferation and impaired cell death contributing to tumor growth. In neurodegenerative conditions HDAC3 interacts with proteins like tau potentially influencing tau-related pathologies. HDAC3 inhibitors are under investigation for therapeutic approaches to these challenging medical conditions highlighting its relevance in disease management.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates (PubMed : 21030595, PubMed : 21444723, PubMed : 23911289, PubMed : 25301942, PubMed : 28167758, PubMed : 28497810, PubMed : 32404892, PubMed : 22230954). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 23911289). Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity (PubMed : 23911289, PubMed : 22230954). Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression (PubMed : 23911289). Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed : 25190803). Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1 (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver (By similarity). Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3 (PubMed : 15653507, PubMed : 21030595, PubMed : 21444723, PubMed : 25301942, PubMed : 28167758). Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl), 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) and isonicotinyl acyl groups from lysine residues, leading to protein decrotonylation, delactylation, de-2-hydroxyisobutyrylation and deisonicotinylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 34608293, PubMed : 34545082, PubMed : 35044827). Catalyzes decrotonylation of MAPRE1/EB1 (PubMed : 34608293). Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 38961290).

See full target information HDAC3

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文献 (92)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:275 PubMed40877230

2025

The deacetylases HDAC1/HDAC2 control JAK2-STAT signaling through the ubiquitin ligase SIAH2.

Applications

Unspecified application

Species

Unspecified reactive species

Al-Hassan M Mustafa,Giuseppe Petrosino,Marten A Fischer,Tina M Schnöder,Désirée Gül,Yanira Zeyn,Christoph Hieber,Johanna Lossa,Sabine Muth,Markus P Radsak,Walburgis Brenner,Markus Christmann,Matthias Bros,Florian H Heidel,Oliver H Krämer

Communications biology 8:1209 PubMed40804340

2025

rTMS ameliorates cerebral ischemia-reperfusion injury by inhibiting Golgi apparatus stress through epigenetic modulation of Gli2.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjiao Zhu,Yongmei Fan,Wenna Peng

Respiratory research 26:214 PubMed40506719

2025

Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Dai,Liling Huang,Lin Li,Le Tang,Yuankai Shi,Xiaohong Han

Science advances 11:eadq2881 PubMed40009679

2025

Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Tang,Yufan Zheng,Guanshuo Hu,Ying Xin,Keming Li,Cunyu Zhang,Xi Chen,Bai Zhang,Xueyi Li,Bing Hu,Qiong Jia,Yong-Ping Zheng,Mo Yang,Youhua Tan

Experimental oncology 46:393-401 PubMed39985345

2025

The Potential Role of HDAC1 and HDAC3 Immunoexpression in P53 Downregulation and Tumor Aggressiveness of Colon and Rectum Carcinomas Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yasir B Qaddoori,Ahmed S K Al-Khafaji,Basim M Khashman,Kifah H Abdulghafour

Nature communications 16:616 PubMed39805830

2025

CPSF6-RARγ interacts with histone deacetylase 3 to promote myeloid transformation in RARG-fusion acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhui Liu,Tanzhen Wang,Lijuan Qi,Yujie Liu,Meng Shan,Fuqiang Wang,Yanglan Fang,Sining Liu,Lijun Wen,Suning Chen,Depei Wu,Yang Xu

Theranostics 15:632-655 PubMed39744685

2025

Orchestrating the frontline: HDAC3-miKO recruits macrophage reinforcements for accelerated myelin debris clearance after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Li,Chenran Wang,Yue Zhang,Yichen Huang,Ziyu Shi,Yuwen Zhang,Yana Wang,Shuning Chen,Yiwen Yuan,He Wang,Leilei Mao,Yanqin Gao

Pain reports 9:e1209 PubMed39512583

2024

Upregulated spinal histone deacetylases induce nociceptive sensitization by inhibiting the GABA system in chronic constriction injury-induced neuropathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Nan-Fu Chen,Hao-Jung Cheng,Hsiao-Mei Kuo,Pei-Yu Chen,Chien-Wei Feng,Zhi-Kang Yao,Wu-Fu Chen,Chun-Sung Sung

Acta physiologica (Oxford, England) 240:e14201 PubMed39007513

2024

Endothelial histone deacetylase 1 activity impairs kidney microvascular NO signaling in rats fed a high-salt diet.

Applications

Unspecified application

Species

Unspecified reactive species

Luke S Dunaway,Anthony K Cook,Cailin E Kellum,Claudia Edell,Davide Botta,Patrick A Molina,Randee S Sedaka,Livius V d'Uscio,Zvonimir S Katusic,David M Pollock,Edward W Inscho,Jennifer S Pollock

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307804 PubMed38837887

2024

Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Hui Li,Shao-Bo Liang,Qi-Wei Huang,Zhen-Zhen Zhou,Zhan Ding,Ni Long,Kwang-Chon Wi,Liang Li,Xi-Ping Jiang,Yu-Jie Fan,Yong-Zhen Xu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC3 抗体 [Y415]

Anti-HDAC3 antibody [Y415]

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369)

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369)

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (AB32369)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369)

Immunoprecipitation - Anti-HDAC3 antibody [Y415] (AB32369)

Western blot - Anti-HDAC3 antibody [Y415] (AB32369)

Western blot - Anti-HDAC3 antibody [Y415] (AB32369)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (92)

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Abcam product promise