Anti-HDAC3 抗体 [3G6] - C-terminal (ab170199)
![Western blot - Anti-HDAC3 antibody [3G6] - C-terminal (ab170199)](https://www.abcam.co.jp/ps/products/170/ab170199/Images/ab170199-169490-anti-hdac3-antibody-3g6-c-terminal-western-blot.jpg "Western blot - Anti-HDAC3 antibody [3G6] - C-terminal (ab170199)")
Key features and details
- Mouse monoclonal [3G6] to HDAC3 - C-terminal
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-HDAC3 antibody [3G6] - C-terminal
HDAC3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3G6] to HDAC3 - C-terminal -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human HDAC3 aa 411-428 (C terminal).
Sequence:NEFYDGDHDNDKESDVEI
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ポジティブ･コントロール
- mouse NIH 3T3 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3G6 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/100 - 1/800. Predicted molecular weight: 48 kDa.
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| 特記事項 |
|---|
|
WB
1/100 - 1/800. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
Sumoylated in vitro. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 15183 Mouse
- SwissProt: O88895 Mouse
- Unigene: 20521 Mouse
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別名
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
see all
画像
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Western blot - Anti-HDAC3 antibody [3G6] - C-terminal (ab170199)Lane 1 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/100 dilution
Lane 2 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/200 dilution
Lane 3 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/400 dilution
All lanes : NIH 3T3 cell lysate
Predicted band size: 48 kDa
Exposure time: 30 secondsNIH 3T3 cell lysate was separated by 10% SDS PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C O/N with ab170199.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab170199 は論文での使用が確認できていません。