Anti-HDAC11/HD11 抗体 [EPR26890-83] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

HDAC11/HD11 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MEF (mouse embryo fibroblast) whole cell lysate
Lane 2 : ab315883 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315883 in MEF whole cell lysate

All lanes:

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (<a href='/products/primary-antibodies/hdac11-hd11-antibody-epr26890-83-ab315883'>ab315883</a>) at 1/30 dilution

All lanes:

MEF (mouse embryo fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

HDAC11/HD11 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate
Lane 2 : ab315883 IP in Mouse brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315883 in mouse brain tissue lysate

All lanes:

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (<a href='/products/primary-antibodies/hdac11-hd11-antibody-epr26890-83-ab315883'>ab315883</a>) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• WB

Supplier Data

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)

This data was developed using ab315883, the same antibody clone in a different buffer formulation.

Low expression : liver, spleen (PMID : 11948178)

The identity of the lower MW band below 39kDa is unknow.

The identity of the higher MW band at approximately 200kDa in lane 1 and lane 4 is unknown.

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (<a href='/products/primary-antibodies/hdac11-hd11-antibody-epr26890-83-ab315883'>ab315883</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Lane 7:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa,36 kDa

false

Exposure time: 180s