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AB316285

Anti-HCoV-229E Nucleocapsid protein 抗体 [abd210]

Anti-HCoV-229E Nucleocapsid protein antibody [abd210]

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Human Recombinant Monoclonal NCAP antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Human coronavirus 229E, Recombinant fragment - Human coronavirus 229E samples.

別名を表示する

7, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] (AB316285)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] (AB316285)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with a HCoV-229E Nucleocapsid protein expression vector containing a myc tag. cells labelling HCoV-229E Nucleocapsid protein with ab316285 at 1/200 (4.985 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with HCoV-229E Nucleocapsid protein expression vector containing a myc-His-tag®. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] (AB316285)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] (AB316285)

Indirect ELISA analysis of ab316285 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : HCoV-229E Nucleocapsid protein,HCoV-NL63 Nucleocapsid protein,SARS-CoV Nucleocapsid protein,HCoV-OC43 Nucleocapsid protein,HCoV-HKU1 Nucleocapsid protein,MERS-CoV Nucleocapsid protein.

Antigen concentration : 5000 ng/ml

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free

Key facts

宿主種

Human

クローン性

Monoclonal

クローン番号

abd210

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human coronavirus 229E

アプリケーション

ICC/IF, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody does not cross-react with recombinant Human coronavirus NL63 nucleocapsid, MERS-CoV nucleocapsid, SARS-CoV nucleocapsid, Human coronavirus OC43 nucleocapsid and Human coronavirus HKU1-Cov nucleocapsid.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human coronavirus 229E": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human coronavirus 229E": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2000 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Human coronavirus 229E": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The HCoV-229E Nucleocapsid protein also known as the N protein plays a mechanical role within the human coronavirus 229E (HCoV-229E). This protein has a mass of around 45 kDa and is expressed in the cytoplasm of infected host cells. Its primary function lies in binding to the viral RNA genome forming a ribonucleoprotein complex that protects the RNA and contributes to the stability of the viral genome. The HCoV-229E nucleocapsid protein is important for the correct assembly and packaging of the viral RNA into new virions.
Biological function summary

The N protein directly impacts viral replication and transcription. It forms part of a larger ribonucleoprotein complex that organizes the viral genome into a functional and protected structure. This complex interacts with other viral proteins to facilitate the replication process. The N protein also boosts the efficiency of the viral RdRp (RNA-dependent RNA polymerase) in transcribing the RNA genome ensuring that viral replication proceeds efficiently and effectively within host cells.

Pathways

The N protein integrates into the coronavirus replication-transcription complex. It coordinates with the viral RdRp and other non-structural proteins of the virus to ensure a streamlined replication process. This protein interacts with cellular pathways that include stress response and immune pathways potentially modulating host immune responses to benefit viral survival and replication. These interactions with host cell pathways may influence the efficiency of viral replication and the pathogenicity of the virus.

HCoV-229E Nucleocapsid protein connects to common cold manifestations and in some cases exacerbates chronic respiratory disorders. The N protein’s interactions with other viral proteins like the Spike protein might enhance viral entry and infectivity which could contribute to varying severity of infection. Understanding the role of the N protein in these contexts is important for developing therapeutic strategies and improving disease management for infections caused by HCoV-229E.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
See full target information N
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Abcam product promise

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