Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナント

Anti-HCN4 抗体 [N114/10] - BSA and Azide free (ab302676)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
  • Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [N114/10] to HCN4 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-HCN4 antibody [N114/10] - BSA and Azide free
    HCN4 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [N114/10] to HCN4 - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF,IHC-Fr or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat thalamus, mouse brain, FFPE tissue sections

  • 特記事項

    ab302676 is a carrier free version of ab302675.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302676の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF,IHC-Fr or WB.

ターゲット情報

  • 関連性

    HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.

  • 細胞内局在

    Membrane; multi pass membrane protein.

  • 参照データベース

    • 別名

      • HCN 4 antibody
      • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
      • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

      This data was developed using ab302675, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse thalamus tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse thalamus. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

      This data was developed using ab302675, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse brain. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

      This data was developed using ab302675, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat thalamus tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on rat thalamus. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

      This data was developed using ab302675, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat liver tissue to label HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Negative control: No staining on rat liver. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)
      Anti-HCN4 antibody [N114/10] - BSA and Azide free (AB302676)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302676 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302676 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab302676.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com