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リコンビナント

Anti-HCN4 抗体 [N114/10] (ab302675)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
  • Anti-HCN4 antibody [N114/10] (AB302675)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [N114/10] to HCN4
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HCN4 antibody [N114/10]
    HCN4 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [N114/10] to HCN4

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF,IHC-Fr or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat thalamus, mouse brain, FFPE tissue sections

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302675の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF,IHC-Fr or WB.

ターゲット情報

  • 関連性

    HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.

  • 細胞内局在

    Membrane; multi pass membrane protein.

  • 参照データベース

    • 別名

      • HCN 4 antibody
      • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
      • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)

      Immunohistochemical analysis of paraffin-embedded mouse thalamus tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse thalamus. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)

      Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse brain. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)

      Immunohistochemical analysis of paraffin-embedded rat thalamus tissue labeling HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on rat thalamus. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue to label HCN4 with ab302675 at 1/500 dilution (2.084 µg/mL) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Negative control: No staining on rat liver. The section was incubated with ab302675 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-HCN4 antibody [N114/10] (AB302675)
      Anti-HCN4 antibody [N114/10] (AB302675)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302675 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302675 は論文での使用が確認できていません。

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