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リコンビナントRabMAb

Anti-HCN4 抗体 [EPR25291-44] (ab289962)

Submit a review Submit a question References (1)
Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
  • Anti-HCN4 antibody [EPR25291-44] (ab289962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25291-44] to HCN4
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HCN4 antibody [EPR25291-44]
    HCN4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25291-44] to HCN4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse heart and brain tissue; Rat heart and brain tissue. IHC-Fr: Mouse heart (fresh) tissue; rat heart (fresh) tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289962の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 関連性

    HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.

  • 細胞内局在

    Membrane; multi pass membrane protein.

  • 参照データベース

    • 別名

      • HCN 4 antibody
      • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
      • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

    画像

    • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling HCN4 with ab289962 at 1/50 (8.96 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on sinoatrial node of mouse heart is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T cells labelling HCN4 with ab289962 at 1/500  followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a HCN4 expression vector, no staining on (B) HEK-293T cells transfected with empty vector. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling HCN4 with ab289962 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on sinoatrial node of rat heart is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded Rat brain (coronal section) tissue labelling HCN4 with ab289962 at 1/100 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse thalamus and corpus striatum. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x400) microscopic images. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded Rat heart tissue labelling HCN4 with ab289962 at 1/100 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat sinoatrial node. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x400) microscopic images. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded Mouse brain (coronal section) tissue labelling HCN4 with ab289962 at 1/100 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse corpus striatum. (A) Low-powered (magnification, x20) and (B) high-powered (magnification, x400) microscopic images. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded Mouse heart tissue labelling HCN4 with ab289962 at 1/100 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse sinoatrial node. (A) Low-powered (magnification, x40) and (B) high-powered (magnification, x400) microscopic images. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling HCN4 with ab289962 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control (PMID: 10430953). No staining on rat liver. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Frozen sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling HCN4 with ab289962 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control (PMID: 10430953). No staining on mouse liver. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

      .

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [EPR25291-44] (ab289962)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling HCN4 with ab289962 at 1/100 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab289962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Anti-HCN4 antibody [EPR25291-44] (ab289962)
      Anti-HCN4 antibody [EPR25291-44] (ab289962)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab289962 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289962 は 1 報の論文で使用されています。

    • Xu P  et al. G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region. Mol Med Rep 27:N/A (2023). PubMed: 36484369

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