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リコンビナント

Anti-HCN1 抗体 [S70] (ab84816)

Reviews (1) Submit a question References (18)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)
  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)
  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)
  • Flow Cytometry - Anti-HCN1 antibody [S70] (ab84816)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)
  • Western blot - Anti-HCN1 antibody [S70] (ab84816)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [S70] to HCN1
  • Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-HCN1 antibody [S70]
    HCN1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [S70] to HCN1

  • 由来種

    Mouse

  • 特異性

    No cross reactivity against HCN2

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, WB, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Rat HCN1 aa 750-950 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9JKB0

  • ポジティブ･コントロール

    • WB: Rat brain lysate. IHC-P: Mouse cerebellum tissue, mouse brain tissue, human brain tissue. ICC: Human hippocampus tissue.

  • 特記事項

    The clone number has been updated from S70-28 to N70/28, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab84816の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.

  • 組織特異性

    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.

  • 配列類似性

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.

  • ドメイン

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed mouse cerebellum tissue labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Cytoplasmic staining of Purkinje cells. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)
    Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed human frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Neurons. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)
    Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed mouse frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Flow Cytometry - Anti-HCN1 antibody [S70] (ab84816)
    Flow Cytometry - Anti-HCN1 antibody [S70] (ab84816)
    Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemical analysis of paraffin-embedded (Bouin’s Fixative - picric acid, acetic acid and formaldehyde) human hippocampus tissue labelling HCN1 with ab84816 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) at 1/50 dilution, for 1 hour at RT. Membrane and cytosolic staining on human hippocampus tissue is observed.

  • Western blot - Anti-HCN1 antibody [S70] (ab84816)
    Western blot - Anti-HCN1 antibody [S70] (ab84816)
    Anti-HCN1 antibody [S70] (ab84816) at 1/1000 dilution + Rat brain membrane lysates at 15 µg

    Secondary
    HRP-Sheep Anti-Mouse IgG antibody

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (18)

ab84816 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab84816 は 18 報の論文で使用されています。

  • Lei X  et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
  • Luo XD  et al. Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel. Neural Regen Res 18:594-602 (2023). PubMed: 36018183
  • Kanemaru K  et al. Spatially resolved multiomics of human cardiac niches. Nature 619:801-810 (2023). PubMed: 37438528
  • Lin W  et al. Downregulation of hyperpolarization-activated cyclic nucleotide-gated channels (HCN) in the hippocampus of patients with medial temporal lobe epilepsy and hippocampal sclerosis (MTLE-HS). Hippocampus 30:1112-1126 (2020). PubMed: 32543742
  • McClements ME  et al. An AAV Dual Vector Strategy Ameliorates the Stargardt Phenotype in Adult Abca4-/- Mice. Hum Gene Ther N/A:N/A (2018). PubMed: 30381971
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Immunocytochemistry/ Immunofluorescence abreview for Anti-HCN1 antibody [S70-28]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cardiac cell line)
Specification
HL-1 cardiac cell line
Fixative
Methanol
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Nov 15 2010

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