Anti-Hck (phospho Y410) 抗体 (ab61055)
Key features and details
- Rabbit polyclonal to Hck (phospho Y410)
- Suitable for: ELISA, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hck (phospho Y410) antibody
Hck 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hck (phospho Y410) -
由来種
Rabbit -
特異性
Detects target only when phosphorylated at tyrosine 410. -
アプリケーション
適用あり: ELISA, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide within Human Hck aa 350-450 (phospho Y410). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P08631 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
1/500 - 1/1000.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils. -
組織特異性
Expressed predominantly in cells of the myeloid and B-lymphoid lineages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates binding to HIV-1 Nef. -
細胞内局在
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3. - Information by UniProt
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参照データベース
- Entrez Gene: 3055 Human
- Entrez Gene: 15162 Mouse
- Entrez Gene: 25734 Rat
- Omim: 142370 Human
- SwissProt: P08631 Human
- SwissProt: P08103 Mouse
- SwissProt: P50545 Rat
- Unigene: 655210 Human
see all -
製品の状態
This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- Bmk antibody
- Hck 1 antibody
- Hck antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Hck (phospho Y410) antibody (ab61055)
1/500-1/1000 ab61055 staining phosphorylated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab61055 は 5 報の論文で使用されています。
- Kim H et al. IgSF11-mediated phosphorylation of pyruvate kinase M2 regulates osteoclast differentiation and prevents pathological bone loss. Bone Res 11:17 (2023). PubMed: 36928396
- Chen M et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. Nat Commun 14:4297 (2023). PubMed: 37463911
- Roversi FM et al. Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis. Front Cell Dev Biol 9:634044 (2021). PubMed: 33842460
- Kao TI et al. Bletinib ameliorates neutrophilic inflammation and lung injury by inhibiting Src family kinase phosphorylation and activity. Br J Pharmacol 178:4069-4084 (2021). PubMed: 34131920
- Poh AR et al. Inhibition of Hematopoietic Cell Kinase Activity Suppresses Myeloid Cell-Mediated Colon Cancer Progression. Cancer Cell 31:563-575.e5 (2017). PubMed: 28399411