Anti-Hck 抗体 [EP2415Y] - BSA and Azide free (ab284722)
![Western blot - Anti-Hck antibody [EP2415Y] - BSA and Azide free (ab284722)](https://www.abcam.co.jp/ps/products/284/ab284722/Images/ab284722-490124-anti-hck-antibody-ep2415y-western-blot-human-bone-marrow.jpg "Western blot - Anti-Hck antibody [EP2415Y] - BSA and Azide free (ab284722)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2415Y] to Hck - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hck antibody [EP2415Y] - BSA and Azide free
Hck 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2415Y] to Hck - BSA and Azide free -
由来種
Rabbit -
特異性
This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: TF-1 and U-937 whole cell lysates.
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特記事項
ab284722 is the carrier-free version of ab75839.
This product has switched from a hybridoma to recombinant production method on 13th September 2022.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2415Y -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284722の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
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| 特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils. -
組織特異性
Expressed predominantly in cells of the myeloid and B-lymphoid lineages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates binding to HIV-1 Nef. -
細胞内局在
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3. - Information by UniProt
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参照データベース
- Entrez Gene: 3055 Human
- Omim: 142370 Human
- SwissProt: P08631 Human
- Unigene: 655210 Human
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製品の状態
This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- Bmk antibody
- Hck 1 antibody
- Hck antibody
see all
画像
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Western blot - Anti-Hck antibody [EP2415Y] - BSA and Azide free (ab284722)All lanes : Anti-Hck antibody [EP2415Y] (ab75839) at 1/20000 dilution
Lane 1 : TF-1 (Human bone marrow erythroblast) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second (Lane 1); 3 seconds (Lane 2).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab284722 は論文での使用が確認できていません。
