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リコンビナントRabMAb

Anti-Hck 抗体 [EP2415Y] (ab75839)

Submit a review Q&A (1)References (3)
Western blot - Anti-Hck antibody [EP2415Y] (ab75839)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EP2415Y] to Hck
    • Suitable for: WB
    • Reacts with: Human

    Conjugates logo Related conjugates and formulations

    Carrier Free

    製品の概要

    • 製品名

      Anti-Hck antibody [EP2415Y]
      Hck 一次抗体 製品一覧

    • 製品の詳細

      Rabbit monoclonal [EP2415Y] to Hck

    • 由来種

      Rabbit

    • 特異性

      This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • ポジティブ･コントロール

      • WB: TF-1 and U-937 whole cell lysates.

    • 特記事項

      This product has switched from a hybridoma to recombinant production method on 13th September 2022.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab75839の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/20000. Predicted molecular weight: 59 kDa.
    特記事項
    WB
    1/20000. Predicted molecular weight: 59 kDa.

    ターゲット情報

    • 機能

      May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.

    • 組織特異性

      Expressed predominantly in cells of the myeloid and B-lymphoid lineages.

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • ドメイン

      The SH3 domain mediates binding to HIV-1 Nef.

    • 細胞内局在

      Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
    • Information by UniProt

    • 参照データベース

      • 製品の状態

        This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

      • 別名

        • Bmk antibody
        • Hck 1 antibody
        • Hck antibody
        • HCK_HUMAN antibody
        • Hemopoietic cell kinase antibody
        • JTK9 antibody
        • p59-HCK/p60-HCK antibody
        • p59Hck antibody
        • p59HCK/p60HCK antibody
        • p61Hck antibody
        • Tyrosine protein kinase HCK antibody
        • Tyrosine-protein kinase HCK antibody
        see all

      画像

      • Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        All lanes : Anti-Hck antibody [EP2415Y] (ab75839) at 1/20000 dilution

        Lane 1 : TF-1 (Human bone marrow erythroblast) whole cell lysate
        Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 59 kDa
        Observed band size: 59 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second (Lane 1); 3 seconds (Lane 2).

      参考文献 (3)

      ab75839 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab75839 は 3 報の論文で使用されています。

      • Sakoda T  et al. TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia. Blood Adv 7:2053-2065 (2023). PubMed: 36745103
      • Chen M  et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. Nat Commun 14:4297 (2023). PubMed: 37463911
      • Roversi FM  et al. Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis. Front Cell Dev Biol 9:634044 (2021). PubMed: 33842460

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      This antibody (cat #2254-1) looks highly interesting. However, as the C-terminal region of the protein shares homology with other SRC-related tyrosine kinases, I wonder whether specificity against HCK vs SRC, LCK, YES, LYN, FYN, FGR and BLK has been tested. Can you provide me this information?  

      Read More
      Answer

      We have not tested ab75839 in-house for cross reactivity with LCK,YES, or BLK so we cannot say for certain whether this antibody would be suitable for detecting these targets. However, in our WB tests we have only observed a single band at ~ 60 KDa when testing the antibody within our recommended dilution range of 1:20,000-200,000. There was one instance when we tested a much higher concentration (1:1,000) and observed an additional band at ~ 140 KDa but it was likely non-specific.

      Read More

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