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リコンビナントRabMAb

Anti-HCF-1 / Host Cell Factor C1 抗体 [EPR25290-18] - BSA and Azide free (ab290000)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
  • Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25290-18] to HCF-1 / Host Cell Factor C1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free
    HCF-1 / Host Cell Factor C1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25290-18] to HCF-1 / Host Cell Factor C1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, K562, C2C12 and C6 whole cell lysates; Mouse lung and testis tissue lysates. IHC-P: Human liver tissue; Rat liver tissue; Mouse lung tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow cyt: HeLa and NIH/3T3 cells.

  • 特記事項

    ab290000 is the carrier-free version of ab289975

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290000の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 208 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 208 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.

  • 組織特異性

    Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.

  • 配列類似性

    Contains 5 Kelch repeats.

  • ドメイン

    The HCF repeat is a highly specific proteolytic cleavage signal.
    The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.

  • 翻訳後修飾

    Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
    O-glycosylated.
    Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.

  • 細胞内局在

    Cytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C1 factor antibody
    • CFF antibody
    • HCF 1 antibody
    • HCF antibody
    • HCF C-terminal chain 6 antibody
    • HCF-1 antibody
    • HCF1 antibody
    • Hcfc1 antibody
    • HCFC1_HUMAN antibody
    • HFC1 antibody
    • Host cell factor 1 antibody
    • Host cell factor antibody
    • Host cell factor C1 (VP16 accessory protein) antibody
    • Host cell factor C1 antibody
    • MGC70925 antibody
    • MRX3 antibody
    • VCAF antibody
    • VP16 accessory protein antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/10000 dilution followed by  Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat liver. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling HCF-1 / Host Cell Factor C1 with ab289975 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution(Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    All lanes : Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] (ab289975) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : C2C12 (mouse myoblasts), whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 7 : Mouse lung tissue lysate
    Lane 8 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 100-150,300 kDa why is the actual band size different from the predicted?



    This data was developed using ab289975, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31693902)

    Exposure times: Lanes 1-4: 6 seconds Lanes 5-6: 26 seconds Lanes 7-8: 8 seconds

  • Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling HCF-1 / Host Cell Factor C1 with ab289975 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubµle Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Lane 1 : Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] (ab289975) at 1/1000 dilution
    Lane 2 : Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] (ab289975) at 1/5000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
    Lane 2 : HeLa transfected with HCF-1/Host Cell Factor C1 siRNA, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 100-150,300 kDa why is the actual band size different from the predicted?



    This data was developed using ab289975, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:Â 31693902).

    Exposure time: 26 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/10000 followed by  Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse lung. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

    This data was developed using ab289975, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/5000  followed by  Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)
    Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (ab290000)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab290000 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab290000 は論文での使用が確認できていません。

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