Anti-HCC1 抗体 (ab25801)
Key features and details
- Rabbit polyclonal to HCC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HCC1 antibody
HCC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HCC1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human HCC1 aa 325-375. The exact sequence is proprietary.
Database link: Q14498 -
エピトープ
The epitope recognized by this antibody maps to a region between residues 325 and 375 of human HCC1. -
ポジティブ・コントロール
- IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HCC1 in HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 60 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 60 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1 (By similarity). May be involved in pre-mRNA splicing process. -
組織特異性
Widely expressed. Highly expressed in pancreas, skeletal muscle, lung and brain. Expressed at intermediate level in kidney, liver and heart. -
配列類似性
Belongs to the splicing factor SR family.
Contains 3 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus speckle. Concentrated in nuclear speckles. Colocalizes with the core spliceosomal snRNP proteins. - Information by UniProt
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参照データベース
- Entrez Gene: 9584 Human
- Omim: 604739 Human
- SwissProt: Q14498 Human
- Unigene: 282901 Human
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別名
- CAPER alpha antibody
- CAPER antibody
- CAPERalpha antibody
see all
画像
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All lanes : Anti-HCC1 antibody (ab25801) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 60 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HCC1 with ab25801 at a 1/5000 dilution in immunohistochemical analysis. DAB staining.
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HCC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, with ab25801 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab25801 at 1 µg/ml.
Lane 1: ab25801 used for IP at 6 µg per reaction (IP by a previous lot of this antibody)
Lane 2: ab25801 used for IP at 6 µg per reaction
Lane 3: Control IgG.
Chemiluminescence detection: 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab25801 は論文での使用が確認できていません。