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リコンビナントRabMAb

Anti-HC-II 抗体 [EPR25618-1] - BSA and Azide free (ab289996)

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Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
  • Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
  • Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
  • Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
  • Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
  • Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25618-1] to HC-II - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt (Intra), ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-HC-II antibody [EPR25618-1] - BSA and Azide free
    HC-II 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25618-1] to HC-II - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Flow Cyt (Intra), ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 whole cell lysate; Human liver tissue lysate. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.

  • 特記事項

    ab289996 is the carrier-free version of ab289971.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25618-1

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289996の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 66-75 kDa (predicted molecular weight: 57 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 66-75 kDa (predicted molecular weight: 57 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
    Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

  • 組織特異性

    Expressed predominantly in liver. Also present in plasma.

  • 関連疾患

    Thrombophilia due to heparin cofactor 2 deficiency

  • 配列類似性

    Belongs to the serpin family.

  • ドメイン

    The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.

  • 翻訳後修飾

    Phosphorylation sites are present in the extracellular medium.
  • Information by UniProt

  • 参照データベース

    • 別名

      • D22S673 antibody
      • HC II antibody
      • HC-II antibody
      • HC2 antibody
      • HCF2 antibody
      • HEP2_HUMAN antibody
      • Heparin cofactor 2 antibody
      • Heparin cofactor II antibody
      • HLS2 antibody
      • Leuserpin 2 antibody
      • LS2 antibody
      • Protease inhibitor leuserpin 2 antibody
      • Protease inhibitor leuserpin-2 antibody
      • serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1 antibody
      • Serpin D1 antibody
      • serpin peptidase inhibitor, clade D (heparin cofactor), member 1 antibody
      • SERPIND1 antibody
      • THPH10 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)

      This data was developed using ab289971, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permebilized HepG2 cells labelling HC-II with ab289971 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)

      This data was developed using ab289971, the same antibody clone in a different buffer formulation.

      HC-II was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab289971 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289971 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

      Lane 2: ab289971 IP in HepG2 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289971 in HepG2 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds

       

    • Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)

      This data was developed using ab289971, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling HC-II with ab289971 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      All lanes : Anti-HC-II antibody [EPR25618-1] (ab289971) at 1/1000 dilution

      Lane 1 : Human liver tissue lysate(RIPA)
      Lane 2 : Human liver tissue lysate(HOT)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 66-75 kDa why is the actual band size different from the predicted?



      This data was developed using ab289971, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 15 seconds

    • Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Western blot - Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      All lanes : Anti-HC-II antibody [EPR25618-1] (ab289971) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : HepG2 transfected with HC-II siRNA, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 57 kDa
      Observed band size: 66-75 kDa why is the actual band size different from the predicted?



      This data was developed using ab289971, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      All these lysates are RIPA buffer.

      Exposure time: 59 seconds

       

    • Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)
      Anti-HC-II antibody [EPR25618-1] - BSA and Azide free (ab289996)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289996 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289996 は論文での使用が確認できていません。

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