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Anti-HBEGF/DTR 抗体 [4G10] (ab66792)

Submit a review Submit a question References (5)
Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792)
  • Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)
  • Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (ab66792)

Key features and details

  • Mouse monoclonal [4G10] to HBEGF/DTR
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin

製品の概要

  • 製品名

    Anti-HBEGF/DTR antibody [4G10]
    HBEGF/DTR 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4G10] to HBEGF/DTR

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IP, WB, ICC/IFmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant fragment corresponding to Human HBEGF/DTR.

  • エピトープ

    EGF domain

  • ポジティブ･コントロール

    • Vero cell extract carrrying human HBEGF/DTR expression vector.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab66792の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.

Use at 2µg/ml
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa).
ICC/IF
Use a concentration of 5 - 10 µg/ml.
特記事項
IP
Use at an assay dependent concentration.

Use at 2µg/ml
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa).
ICC/IF
Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

  • 配列類似性

    Contains 1 EGF-like domain.

  • 翻訳後修飾

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).

  • 細胞内局在

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt

  • 参照データベース

    • 別名

      • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
      • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
      • Diphtheria toxin receptor antibody
      • DT R antibody
      • DT-R antibody
      • DTR antibody
      • DTS antibody
      • DTSF antibody
      • HB-EGF antibody
      • HBEGF antibody
      • HBEGF_HUMAN antibody
      • HEGFL antibody
      • Heparin binding EGF like growth factor antibody
      • Heparin binding epidermal growth factor antibody
      • Heparin binding epidermal growth factor like growth factor antibody
      • Heparin-binding EGF-like growth factor antibody
      • Proheparin binding EGF like growth factor antibody
      see all

    画像

    • Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792)
      Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792)
      All lanes : Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/ml

      Lane 1 : Vero (African green monkey kidney epithelial cell) whole cell lysate
      Lane 2 : Vero cell extract from cells carrying human HBEGF/DTR expression vector
      Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector

      Predicted band size: 23, 40 kDa
      Observed band size: 20-28 kDa why is the actual band size different from the predicted?
      Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)
      Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)

      Lane 1: Vero cell extract, 1μg
      Lane 2: Vero cells carrying Human HBEGF/DTR expression vector, 1μg
      Lane 3: Vero Cells carrying mouse HBEGF/DTR expression vector, 1μg

      Ab66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.

    • Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (ab66792)
      Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (ab66792)

      These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

    参考文献 (5)

    ab66792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab66792 は 5 報の論文で使用されています。

    • Arnold AE  et al. Attenuated diphtheria toxin mediates siRNA delivery. Sci Adv 6:N/A (2020). PubMed: 32917630
    • Shen L  et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med 13:3021-3031 (2017). PubMed: 28587375
    • Bæk R  et al. Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma? Transfus Apher Sci 55:44-52 (2016). PubMed: 27470710
    • Tang XH  et al. The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys Res Commun 422:676-80 (2012). Human . PubMed: 22609777
    • Desjardins F  et al. Modulation of the cochaperone AHA1 regulates heat-shock protein 90 and endothelial NO synthase activation by vascular endothelial growth factor. Arterioscler Thromb Vasc Biol 32:2484-92 (2012). PubMed: 22859491

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