AB228709

Anti-HBE 抗体 - N-terminal

Anti-HBE antibody - N-terminal

JP Deleterious:医薬用外劇物

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(2 Publications)

Rabbit Polyclonal HBE antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HBE1.

別名を表示する

HBE, HBE1, Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain

4 Images

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

239T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

15% SDS-PAGE gel.

CFA : Complete Freunds adjuvant

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/500 dilution

Lane 1:

Untreated Mouse leukocyte tissue extract at 30 µg

Lane 2:

CFA-treated mouse leukocyte tissue extract at 30 µg

Predicted band size: 16 kDa

true

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

All lanes:

Mouse spleeen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

All lanes:

Rat spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human HBE1. The exact immunogen used to generate this antibody is proprietary information.

P02100

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The HBE protein also known as hepatitis B virus e antigen (HBeAg) displays significant mechanical functions in the viral life cycle. It has a mass of approximately 17–25 kDa and expresses mainly in the cells of the liver during hepatitis B virus replication. HBE gets produced during the conversion of the precore protein into its mature form which allows it to function effectively as a viral antigen. 'Anti-HBE' and 'anti-HBeAg' antibodies target this antigen. The protein plays a role in immune tolerance mechanisms by modulating host immune responses against the hepatitis B virus.

Biological function summary

The HBE protein contributes to the persistence of hepatitis B virus infection. It's part of the viral replication complex and helps maintain viral chronicity by repressing immune activation. The presence of HBE in the host leads to a reduction in the recognition of infected hepatocytes by the immune system allowing the virus to persist longer. Identifying the presence of 'HBE antigen' and 'anti-HBE' antibodies helps monitor the progression of hepatitis B infection. This antigen is a marker for active viral replication and infectiousness.

Pathways

HBE integrates into the hepatitis B virus life cycle and immune response pathways. It involves the regulation of host immune responses particularly the adaptive immune system's recognition and clearance of infected hepatocytes. HBeAg influences the pathways by interacting with proteins related to immune checkpoints ensuring viral persistence. These interactions alter the balance of pro-inflammatory and anti-inflammatory signals affecting the body's ability to mount an effective immune response against the infected liver cells.

HBE plays an important role in chronic hepatitis B infection and liver disease progression. It associates with reduced inflammation in chronic hepatitis B patients often correlating with lower liver damage. However persistent expression of HBE can contribute to the development of hepatitis B-related liver cirrhosis due to prolonged immune suppression. The production of 'anti-HBE' and 'anti-HBeAg' antibodies against HBE indicates the host's immune status and helps in determining the phase of infection. Understanding the interactions between HBE and other hepatic proteins assists clinicians in making decisions regarding hepatitis B treatment strategies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.

See full target information HBE1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 208:672-684 PubMed35022275

2022

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Li,Xiaoqiong Duan,Chuanlong Zhu,Xiao Liu,Andre J Jeyarajan,Min Xu,Zeng Tu,Qiuju Sheng,Dong Chen,Chuanwu Zhu,Tuo Shao,Zhimeng Cheng,Shadi Salloum,Esperance A Schaefer,Annie J Kruger,Jacinta A Holmes,Raymond T Chung,Wenyu Lin

Molecular cell 81:3128-3144.e7 PubMed34216544

2021

Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cong,Min Peng,Arne Nedergaard Kousholt,Wei Ting C Lee,Silviana Lee,Sumeet Nayak,John Krais,Pamela S VanderVere-Carozza,Katherine S Pawelczak,Jennifer Calvo,Nicholas J Panzarino,John J Turchi,Neil Johnson,Jos Jonkers,Eli Rothenberg,Sharon B Cantor

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HBE 抗体 - N-terminal

Anti-HBE antibody - N-terminal

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise