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リコンビナントRabMAb

Anti-HB9/HLXB9/MNX1 抗体 [EPR3342] (ab79541)

Submit a review Q&A (1)References (7)
Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)
  • Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HB9/HLXB9/MNX1 antibody [EPR3342]
    HB9/HLXB9/MNX1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1

  • 由来種

    Rabbit

  • 特異性

    There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide within Human HB9/HLXB9/MNX1 aa 250-350 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Molt-4 and Raji cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79541の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Putative transcription factor involved in pancreas development and function.

  • 組織特異性

    Expressed in lymphoid and pancreatic tissues.

  • 関連疾患

    Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.

  • 配列類似性

    Contains 1 homeobox DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox 1 antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    画像

    • Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)
      Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)
      All lanes : Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541) at 1/200000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?

    • Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)
      Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)

    参考文献 (7)

    ab79541 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab79541 は 7 報の論文で使用されています。

    • Schaefers C  et al. The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons. Stem Cells Int 2022:1320950 (2022). PubMed: 36530489
    • Zhu B  et al. MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1. Front Oncol 10:1307 (2020). PubMed: 32850410
    • Park S  et al. Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation. Int J Mol Sci 20:N/A (2019). PubMed: 31159418
    • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . PubMed: 22855461
    • Ruggiu M  et al. A role for SMN exon 7 splicing in the selective vulnerability of motor neurons in spinal muscular atrophy. Mol Cell Biol 32:126-38 (2012). PubMed: 22037760
    • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). IHC-P, WB ; Human . PubMed: 21484430
    • Wu CY  et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). PubMed: 21385431

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Thank you for the detailed reply! The other antibody I used is ab79541. I haven’t rule out the secondary yet (which was from Vector labs biotynilation – sheep IgG). Will do this later and also try other options as 1) blocking endogenous biotin…. 2) an enzymatic antigen retrieval…. It will be great if you would send me the protocol by which the antibody (ab59795) was validated? And I wonder if it is possible that you can send me a small amount of the HB9 antibody (ab92606) you mentioned for me to try out? Thank you.

    Read More
    Answer

    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Abpromise guarantee for this application (IHC on paraffin-embedded tissue sections) and this species (human). If I cannot help you obtain a satisfactory result, I will be happy to look into replacing this. However, I am concerned about the possility that this transcription factor is not present in prostate. Do you have any other tissues you can include as apositive control, in particular pancreas?

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