AB181997

Anti-HAPLN1 抗体 [EPR6338]

Name: Anti-HAPLN1 抗体 [EPR6338] (ab181997) | Abcam
Brand: Abcam Limited
SKU: AB181997
Price: 48000 JPY
Availability: InStock
Rating: 3 (3 reviews)

Anti-HAPLN1 antibody [EPR6338]

RabMAb
Recombinant
詳細を見る

(3 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal HAPLN1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

別名を表示する

CRTL1, HAPLN1, Hyaluronan and proteoglycan link protein 1, Cartilage-linking protein 1, Proteoglycan link protein, Cartilage-link protein

5 Images

Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (AB181997)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

All lanes:

Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

All lanes:

Human spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

All lanes:

Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

Caco2 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

Predicted band size: 40 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6338

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HAPLN1 also known as hyaluronan and proteoglycan link protein 1 is an important structural component in the extracellular matrix. This protein links hyaluronan to proteoglycans which contributes to the integrity and function of the matrix. HAPLN1 has a molecular mass of approximately 39 kDa and is expressed in various tissues including cartilage and nervous tissues. Its expression plays a significant role in maintaining the properties of these tissues.

Biological function summary

Hyaluronan and proteoglycan link protein 1 supports the organization and stabilization of the extracellular matrix network. As part of this framework HAPLN1 forms complexes with hyaluronan and aggrecan ensuring structural stability and resilience in tissues experiencing mechanical stress. The association with such complexes impacts cellular signaling and tissue hydration which are important for maintaining tissue homeostasis and function.

Pathways

HAPLN1 participates in extracellular matrix organization and cell signaling processes. It is actively involved in the aggrecan pathway which influences cartilage development and repair. HAPLN1 is also connected to proteins like aggrecan and link proteins that help mediate interactions within the matrix and regulate cellular responses to mechanical stress.

Abnormal expression or function of HAPLN1 has links to osteoarthritis and intervertebral disc degeneration. In these conditions the disruption of extracellular matrix composition alters tissue integrity and function. An imbalance involving HAPLN1 along with proteins like aggrecan contributes to the pathogenesis and progression of these disorders affecting joint and disc health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.

See full target information HAPLN1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 14:335-347 PubMed38618242

2024

Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trapping.

Applications

Unspecified application

Species

Unspecified reactive species

Ding-Jun Hao,Yue Qin,Shi-Jie Zhou,Bu-Huai Dong,Jun-Song Yang,Peng Zou,Li-Ping Wang,Yuan-Ting Zhao

Journal of cellular physiology 237:2469-2477 PubMed35220586

2022

Single-cell RNA sequencing reveals the cell types heterogenicity of human discoid lateral meniscus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Sun,Wenhui Zhang,Fangzhou Liu,Deng Li,Zhiqing Cai,Zhencheng Huang,Meiyi Chen,Zhencan Lin,Jie Xu,Ruofan Ma

Molecular psychiatry 23:2302-2313 PubMed30254235

2018

The hippocampal extracellular matrix regulates pain and memory after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Maral Tajerian,Victor Hung,Huy Nguyen,Gail Lee,Lydia-Marie Joubert,Andrey Victorovich Malkovskiy,Bende Zou,Simon Xie,Ting-Ting Huang,J David Clark

Neuron 99:702-719.e6 PubMed30078576

2018

Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine R Long,Ben Newland,Marta Florio,Nereo Kalebic,Barbara Langen,Anna Kolterer,Pauline Wimberger,Wieland B Huttner

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HAPLN1 抗体 [EPR6338]

Anti-HAPLN1 antibody [EPR6338]

Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise