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Anti-Hairy Cell Leukemia 抗体 [DBA.44], prediluted (ab857)

Submit a review Q&A (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

    Key features and details

    • Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgM

    製品の概要

    • 製品名

      Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted
      Hairy Cell Leukemia 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted

    • 由来種

      Mouse

    • 特異性

      This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Hairy Cell Leukemia. BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C. Do Not Freeze.

    • バッファー

      pH: 7.3
      Preservative: 0.05% Sodium azide

      Inert stabilizer
    • Concentration information loading...

    • ポリ/モノ

      モノクローナル

    • クローン名

      DBA.44

    • ミエローマ

      unknown

    • アイソタイプ

      IgM

    • 軽鎖の種類

      kappa

    • 研究分野

    関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab857の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use at an assay dependent concentration.
    特記事項
    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 細胞内局在

      Nuclear

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

      Immunohistochemistry (Formalin fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Hairy Cell Leukemia with ab857.

    参考文献 (0)

    ab857 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab857 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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    Answer

    Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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