Anti-hair cortex Cytokeratin/K40 抗体 [AE13] (ab16113)
![Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)](https://www.abcam.co.jp/ps/products/16/ab16113/Images/ab16113-24844-anti-hair-cortex-cytokeratin-k40-antibody-ae13-western-blot.jpg "Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)")
Key features and details
- Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40
- Suitable for: IHC-Fr, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-hair cortex Cytokeratin/K40 antibody [AE13]
hair cortex Cytokeratin/K40 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40 -
由来種
Mouse -
特異性
This antibody is specific for the family of hair cortex keratins. -
アプリケーション
適用あり: IHC-Fr, IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Full length native protein (purified) corresponding to Human hair cortex Cytokeratin/K40.
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特記事項
This antibody is an excellent marker for hair and nail differentiation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
This antibody is an excellent marker for hair and nail differentiation. -
ポリ/モノ
モノクローナル -
クローン名
AE13 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (4) |
Use at an assay dependent concentration.
|
| IHC-P | (3) |
Use at an assay dependent concentration.
|
| WB |
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa).
|
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| Flow Cyt |
1/20 - 1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa). |
|
Flow Cyt
1/20 - 1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May play a role in late hair differentiation. -
組織特異性
Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level). -
配列類似性
Belongs to the intermediate filament family. -
発生段階
During differentiation of the hair, it is one of the last keratins expressed. - Information by UniProt
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参照データベース
- Entrez Gene: 125115 Human
- Entrez Gene: 406221 Mouse
- SwissProt: Q6A162 Human
- SwissProt: Q6IFX3 Mouse
- Unigene: 534929 Human
- Unigene: 422372 Mouse
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別名
- CK-40 antibody
- Cytokeratin-40 antibody
- hair cortex Cytokeratin antibody
see all
画像
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Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)All lanes : Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) at 1 µg/ml
Lane 1 :Mouse skin tissue lysate (14 days) (ab7271)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 - 46 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)](https://www.abcam.co.jp/ps/products/16/ab16113/Images/ab16113-24843-anti-hair-cortex-cytokeratin-k40-antibody-ae13-flow-cytometry.jpg)
Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab16113 は 40 報の論文で使用されています。
- Ryu YC et al. CXXC5 Mediates DHT-Induced Androgenetic Alopecia via PGD2. Cells 12:N/A (2023). PubMed: 36831222
- Sibony-Benyamini H et al. Hdac1 and Hdac2 regulate the quiescent state and survival of hair-follicle mesenchymal niche. Nat Commun 14:4820 (2023). PubMed: 37563109
- Wu W et al. Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles. Mol Ther Nucleic Acids 32:94-110 (2023). PubMed: 37020681
- Shi TW et al. Effects of NCSTN Mutation on Hair Follicle Components in Mice. Dermatology 239:60-71 (2023). PubMed: 35843211
- Joseph CB et al. Meta-GWAS Reveals Novel Genetic Variants Associated with Urinary Excretion of Uromodulin. J Am Soc Nephrol 33:511-529 (2022). PubMed: 35228297