Anti-HADHB 抗体 [5C3AB7] (ab110301)
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.co.jp/ps/products/110/ab110301/Images/ab110301-242584-anti-hadhb-antibody-5c3ab7-western-blot.jpg "Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)")
Key features and details
- Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: IP, WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HADHB antibody [5C3AB7]
HADHB 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5C3AB7] to HADHB -
由来種
Mouse -
アプリケーション
適用あり: IP, WB, Flow Cyt, ICCmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HepG2 and HLM cell lysates; HDFn and HeLa cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
ポリ/モノ
モノクローナル -
クローン名
5C3AB7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110301の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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パスウェイ
Lipid metabolism; fatty acid beta-oxidation. -
関連疾患
Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex. -
配列類似性
Belongs to the thiolase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 3032 Human
- Omim: 143450 Human
- SwissProt: P55084 Human
- Unigene: 515848 Human
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別名
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
see all
画像
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Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa -
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.co.jp/ps/products/110/ab110301/Images/ab110301-123552-anti-hadhb-antibody-5c3ab7.gif)
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate
Predicted band size: 51 kDaAs a Western blot antibody, ab110301 works with HepG2 (human) cells.
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![Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.co.jp/ps/products/110/ab110301/Images/ab110301-123551-anti-hadhb-antibody-5c3ab7-immunocytochemistry.jpg)
Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue). -
![Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.co.jp/ps/products/110/ab110301/Images/ab110301-123550-anti-hadhb-antibody-5c3ab7-flow-cytometry.gif)
Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red). -
![Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)](https://www.abcam.co.jp/ps/products/110/ab110301/Images/ab110301-123549-anti-hadhb-antibody-5c3ab7-immunoprecipitation.gif)
Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)As an IP antibody, MS733 works with human samples.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab110301 は 2 報の論文で使用されています。
- Lu J et al. Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma. Front Oncol 11:737127 (2021). PubMed: 34976793
- Li XX et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065