Anti-HA tag 抗体 [HL1603]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (AB308398)

Immunohistochemical analysis of mock and HA tag - transfected HEK293T cell pellet labeling HA tag using ab308398 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (AB308398)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HA tag using ab308398 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-HA tag antibody [HL1603] (AB308398)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [HL1603] (ab308398) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HA tag antibody [HL1603] (ab308398) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• I-ELISA

Supplier Data

Indirect ELISA - Anti-HA tag antibody [HL1603] (AB308398)

Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (ab308398) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1/10000) was used to detect the bound primary antibody.