AB18181

Anti-HA tag 抗体 [HA.C5]

Name: Anti-HA tag 抗体 [HA.C5] (ab18181) | Abcam
Brand: Abcam Limited
SKU: AB18181
Price: 56000 JPY
Availability: InStock
Rating: 4 (19 reviews)

Anti-HA tag antibody [HA.C5]

Lab Essentials
詳細を見る

(19 Reviews)

(340 Publications)

Anti-HA tag antibody [HA.C5] (ab18181) is a mouse monoclonal antibody detecting HA tag in Western Blot, ICC/IF.

- Clone HA.C5 is the most cited clone to HA tag
- Over 260 publications
- Trusted since 2005

別名を表示する

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

5 Images

AbReview39578****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

Lanes 1 - 2:

HEK293 whole cell lysate - transfected at 30 µg

Lane 3:

HEK293 whole cell lysate - untransfected at 30 µg

Secondary

All lanes:

IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 85 kDa

false

Exposure time: 5min

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

ICC/IF

AbReview31652****

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

This image is courtesy of an Abreview submitted by Harendra Chahar

Unknown

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

Predicted band size: 64 kDa

false

AbReview5311****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

Lane 1:

WCE from cell line transfected for HA-tagged protein at 50 µg

Lane 2:

WCE from a cell line transfected with empty vector at 50 µg

Secondary

All lanes:

HRP conjugated Goat anti-mouse IgG (H+L)

Predicted band size: 64 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

HA.C5

アイソタイプ

IgG3

キャリアフリー

アプリケーション

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

Anti-HA tag antibody [HA.C5] (ab18181) is a House Mouse Monoclonal antibody and is validated for use in ICC/IF, WB.

Anti-HA tag antibody [HA.C5] (ab18181) has been cited over 269 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-HA tag antibody [HA.C5] (ab18181) has high sensitivity and specificity.

Anti-HA tag antibody [HA.C5] (ab18181) has 17 independent reviews from customers.

HA tag antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-HA tag antibody [HA.C5] (ab18181) has been validated in ICC/IF, WB

Anti-HA tag antibody [HA.C5] (ab18181) specifically detects HA tag (UniProt ID: P03435; Molecular weight: 62kDa) and is sold in 100 ug selling sizes.

This product was changed from ascites to tissue culture supernatant on 5^th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Purified from TCS

バッファー組成

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information HA tag

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文献 (340)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867552

2025

ENO1 from Disrupts Host Glycolysis and Inflammation by Binding ACTB.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Rui Li,Xiao-Jiao Yu,Jia-Yin Liang,Jin-Liang Sheng,Hui Zhang,Chuang-Fu Chen,Zhong-Chen Ma,Yong Wang

Journal of orthopaedic surgery and research 20:517 PubMed40420098

2025

TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li

Science advances 11:eadt5388 PubMed40333975

2025

Inhibition of placental trophoblast fusion by guanylate-binding protein 5.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Krchlikova,Elisabeth Braun,Johanna Weiss,Krystof Stafl,Lukas Jech,Smitha Srinivasachar Badarinarayan,Rishikesh Lotke,Martin Travnicek,Charlotte Baur,Paul Stark,Isabell Haussmann,Yueshuang Lu,Moritz Petersen,Wen Cui,Wei Wang,Bianca M Fäger,Hannah Reisinger,Kenzo Tokunaga,Oya Cingöz,Konstantin M J Sparrer,Madhuri S Salker,Jiri Hejnar,Frank Kirchhoff,Katerina Trejbalova,Daniel Sauter

Frontiers in cellular and infection microbiology 15:1550545 PubMed40256452

2025

The Effect of glycocholic acid on the growth, membrane permeability, conjugation and antibiotic susceptibility of Enterobacteriaceae.

Applications

Unspecified application

Species

Unspecified reactive species

Bar Piscon,Boris Fichtman,Amnon Harel,Amos Adler,Galia Rahav,Ohad Gal-Mor

Cellular and molecular life sciences : CMLS 82:128 PubMed40128454

2025

The HBV large envelope protein initiates virion assembly by recruiting capsids at membrane rich domains related to late endosome.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Seigneuret,Sébastien Eymieux,Vanessa Sarabia-Vega,Roxane Lemoine,Julien Burlaud-Gaillard,Pierre Raynal,Christophe Hourioux,Camille Sureau,Philippe Roingeard,Hugues de Rocquigny

Nature communications 16:1953 PubMed40000658

2025

RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Huang,Juan Du,Yan Liu,Lu Lu,Yanzhuo Xu,Jianfei Shi,Qing Liu,Qi Li,Yang Liu,Yaqiu Chen,Meng Du,Yiming Zhao,Liangxiao Huo,Weiran Wang,Chenxi Ding,Liya Wei,Jianguo Wu,Yao-Wu Yuan,Jinfeng Chen,Ruixi Li,Feng Cui,Xiaoming Zhang

The EMBO journal 44:1414-1441 PubMed39875724

2025

Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Hua Lu,Yao Liu,Jiabei Wang,Qingan Xia,Xiangmin Shi,Yan Jin,Xiaolin Liang,Wei Wang,Xiaopeng Ma,Yangyi Wang,Meng Gong,Canjun Li,Chunlei Cang,Qinghua Cui,Ceshi Chen,Tao Shen,Lianxin Liu,Xiangting Wang

Molecular & cellular proteomics : MCP 24:100890 PubMed39617063

2024

Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dicle Malaymar Pinar,Helka Göös,Zenglai Tan,Esa-Pekka Kumpula,Iftekhar Chowdhury,Zixian Wang,Qin Zhang,Kari Salokas,Salla Keskitalo,Gong-Hong Wei,Asli Kumbasar,Markku Varjosalo

STAR protocols 5:103454 PubMed39570747

2024

Protocol for detecting and quantifying hATF4-HA in non-stress versus stress conditions using automated and quantitative Jess western blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Anna M Smirnova,Anna Herrmannová,Leoš Shivaya Valášek

Scientific reports 14:21615 PubMed39284887

2024

Mint/X11 PDZ domains from non-bilaterian animals recognize and bind Ca2 calcium channel C-termini in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia N Harracksingh,Anhadvir Singh,Tatiana D Mayorova,Brian Bejoy,Jillian Hornbeck,Wassim Elkhatib,Gregor McEdwards,Julia Gauberg,Abdul Taha,Ishrat Maliha Islam,Ted Erclik,Mark A Currie,Marcus Noyes,Adriano Senatore

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HA tag 抗体 [HA.C5]

Anti-HA tag antibody [HA.C5]

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (340)

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