Anti-HA tag 抗体 [HA-7] (ab49969)
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.co.jp/ps/products/49/ab49969/Images/ab49969-273324-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)")
Key features and details
- Mouse monoclonal [HA-7] to HA tag
- Suitable for: ELISA, ICC/IF, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-HA tag antibody [HA-7]
HA tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HA-7] to HA tag -
由来種
Mouse -
アプリケーション
適用あり: ELISA, ICC/IF, IP, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide:
YPYDVPDYA
conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag -
ポジティブ･コントロール
- Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
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特記事項
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from Tissue culture supernatant”. -
ポリ/モノ
モノクローナル -
クローン名
HA-7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49969の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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| IP | (1) |
Use a concentration of 0.5 - 2 µg/ml.
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| WB | (2) |
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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IP
Use a concentration of 0.5 - 2 µg/ml. |
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WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.co.jp/ps/products/49/ab49969/Images/ab49969-198918-anti-ha-tag-antibody-ha-7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)This image is courtesy of an anonymous Abreviewab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)](https://www.abcam.co.jp/ps/products/49/ab49969/Images/ab49969-273325-anti-ha-tag-antibody-ha-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab49969 は 13 報の論文で使用されています。
- Offley SR et al. A combinatorial approach to uncover an additional Integrator subunit. Cell Rep 42:112244 (2023). PubMed: 36920904
- Antal CE et al. A super-enhancer-regulated RNA-binding protein cascade drives pancreatic cancer. Nat Commun 14:5195 (2023). PubMed: 37673892
- Shi YF et al. Interaction between CD9 and PI3K-p85 activates the PI3K/AKT signaling pathway in B-lineage acute lymphoblastic leukemia. Oncol Rep 46:N/A (2021). PubMed: 34036396
- Oury J et al. Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia. Nature 595:404-408 (2021). PubMed: 34163073
- Harris IS et al. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion. Cell Metab 29:1166-1181.e6 (2019). PubMed: 30799286