Anti-HA tag 抗体 (ab9111)
Key features and details
- Chicken polyclonal to HA tag
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: unknown
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HA tag antibody
HA tag 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to HA tag -
由来種
Chicken -
特異性
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate. -
アプリケーション
適用あり: WB, ELISA, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA (epitope)
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ポジティブ・コントロール
- ICC/IF: CHO cells transfected with 12 tags constructs (CHO-12 tags) and transfected with GFP-HA constructs (CHO-GFP-HA).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading...
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精製度
IgY fraction -
特記事項(精製)
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 4 - 5 µg/ml.
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特記事項 |
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WB
1/1000. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 4 - 5 µg/ml. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilised with 0.1% Triton X-100 in cells transfected with GFP-HA contrsucts (as CHO-GFP-HA) or parental non- transfected CHO cells labelling with chicken anti-HA tag (ab9111) at 5µg/ml, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5µg/ml (Red). Nucleus was counterstained with DAPI (Blue).
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Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling HA tag with ab9111 at 1/250 dilution (4μg/mL), followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 1/500 dilution (2 μg/mL) (Red). Nucleus was counterstained with DAPI (white). Bottom panel images showed Parallel staining in parental CHO cell line.
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Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9111 at 5μg/mL, followed by Goat Anti- Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 μg/mL (Red). Nucleus was counterstained with DAPI (Blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab9111 は 21 報の論文で使用されています。
- Zeballos C MA et al. Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins. Nat Commun 14:6492 (2023). PubMed: 37838698
- Zhao B et al. Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration. Acta Neuropathol Commun 11:200 (2023). PubMed: 38111057
- Wendt L et al. Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1. J Virol 96:e0090022 (2022). PubMed: 36040180
- Powell JE et al. Targeted gene silencing in the nervous system with CRISPR-Cas13. Sci Adv 8:eabk2485 (2022). PubMed: 35044815
- Decombe S et al. Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability. Epigenetics Chromatin 14:35 (2021). PubMed: 34321103