Anti-HA tag 抗体 (ab20084)
Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HA tag antibody
HA tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HA tag -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to HA tag (internal sequence) conjugated to keyhole limpet haemocyanin.
Sequence:YPYDVPDYA
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特記事項
The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20084の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
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ICC/IF | (1) |
Use at an assay dependent dilution.
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WB | (1) |
1/5000. Predicted molecular weight: 1 kDa.
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特記事項 |
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ELISA
1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. |
ICC/IF
Use at an assay dependent dilution. |
WB
1/5000. Predicted molecular weight: 1 kDa. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.
Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab20084 は 17 報の論文で使用されています。
- Vicioso-Mantis M et al. JMJD3 intrinsically disordered region links the 3D-genome structure to TGFβ-dependent transcription activation. Nat Commun 13:3263 (2022). PubMed: 35672304
- Yoon S et al. TGF-ß-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance. Cell Rep 31:107685 (2020). PubMed: 32460012
- Gingell JJ et al. Distinct Patterns of Internalization of Different Calcitonin Gene-Related Peptide Receptors. ACS Pharmacol Transl Sci 3:296-304 (2020). PubMed: 32296769
- Lulla V & Firth AE A hidden gene in astroviruses encodes a viroporin. Nat Commun 11:4070 (2020). PubMed: 32792502
- Cheng Z et al. K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3K18ac through the MDM2-GCN5-SIRT7 axis. Pharm Biol 57:701-709 (2019). PubMed: 31613681