Anti-HA tag 抗体 (ab184643)
Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: WB, IP, ICC, ELISA
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-HA tag antibody
HA tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HA tag -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8 -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184643の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/30000.
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| IP |
Use a concentration of 1 - 4 µg/ml.
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| ICC |
1/100 - 1/400.
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| ELISA |
1/1000 - 1/30000.
for coating plates use a dilution range of 1/1000 - 1/30,000 |
| 特記事項 |
|---|
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WB
1/1000 - 1/30000. |
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IP
Use a concentration of 1 - 4 µg/ml. |
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ICC
1/100 - 1/400. |
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ELISA
1/1000 - 1/30000. for coating plates use a dilution range of 1/1000 - 1/30,000 |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Western blot - Anti-HA tag antibody (ab184643)
Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified rabbit anti-HA tag antibody ab184643 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab184643 は 1 報の論文で使用されています。
- Adamczyk M & Szatkowska R Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS One 12:e0185516 (2017). PubMed: 28961268
