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Anti-HA tag 抗体 (ab13834)

Reviews (3)Q&A (1)References (11)
Western blot - Anti-HA tag antibody (ab13834)

    Key features and details

    • Rabbit polyclonal to HA tag
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-HA tag antibody [EPR4095] (ab182009)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-HA tag antibody
      HA tag 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to HA tag

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Synthetic peptide within HA tag conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      (Peptide available as ab13835)

    • ポジティブ･コントロール

      • WB: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 98.98% PBS, 1% BSA

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13834の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    1/500. Can be blocked with HA tag peptide (ab13835).
    特記事項
    WB
    1/500. Can be blocked with HA tag peptide (ab13835).

    ターゲット情報

    • 関連性

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • 別名

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    画像

    • Western blot - Anti-HA tag antibody (ab13834)
      Western blot - Anti-HA tag antibody (ab13834)
      All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

      Lane 1 : HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
      Lane 2 : HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

    参考文献 (11)

    ab13834 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13834 は 11 報の論文で使用されています。

    • Li T  et al. SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5- and PIF4-mediated signaling. J Integr Plant Biol 64:1706-1723 (2022). PubMed: 35848532
    • Choi C  et al. OsDWD1 E3 ligase-mediated OsNPR1 degradation suppresses basal defense in rice. Plant J 112:966-981 (2022). PubMed: 36168109
    • Lei KH  et al. Crosstalk between CST and RPA regulates RAD51 activity during replication stress. Nat Commun 12:6412 (2021). PubMed: 34741010
    • Wan J  et al. MYB70 modulates seed germination and root system development in Arabidopsis. iScience 24:103228 (2021). PubMed: 34746697
    • Wang C  et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). PubMed: 31076507
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-HA tag antibody

     Inconclusive
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    PROF. Vidita Vaidya

    Verified customer

    投稿 Jul 23 2014

    Western blot abreview for Anti-HA tag antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    40 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Svetlana Khoronenkova

    Verified customer

    投稿 Aug 17 2010

    Western blot abreview for Anti-HA tag antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human glioma cells)
    Loading amount
    250000 cells
    Specification
    Human glioma cells
    Treatment
    Transfected with lipofectamine
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 04 2007

    Question

    Hi Technical,



    We have a customer who is looking for a HA tag antibody and a matching secondary, I have sourced and found him the following;



    https://www.abcam.com/HA-tag-antibody-ChIP-Grade-ab9110.html

    https://www.abcam.com/HA-tag-antibody-ab13834.html

    https://www.abcam.com/HA-tag-antibody-Agarose-ab27029.html

    https://www.abcam.com/HA-tag-antibody-ChIP-Grade-ab71113.html

    https://www.abcam.com/Proteinase-K-ab64220.html



    The customer’s first email;

    I received your email address from a colleagues at the school of biology sciences. I am looking for some things that you probably sell. First of all an rabbit anti-HA antibody and an anti-rabbit FITC-conjugated antibody (I think they are both from Abcam). And secondly proteinase K. Do you sell these and can you give me a quotation for each of them?



    But as he is new to this, he has provide the following details, as he didn’t provide much in the first place.

    The customer’s Reply;

    Since I’m relatively new to this field of research (I do more behaviour than immunohistochemistry), I’m not entirely sure which of the antibodies is the best. However, perhaps I can explain the experiment and you might know better . We are currently transfecting HEK cells with a mutant receptor that has an HA tag and we want to see whether the receptor is nicely incorporated into the membrane or whether it is somewhere else (using confocal laser microscopy). So we need a good anti-HA antibody for that (and indeed the anti-rabbit conjugated antibody would then be the secondary antibody. I hope that help.

    Are you able to help me out with this, as it is out my field?



    Looking forward to your response.





    Kind regards,



    Melissa Gliddon

    Customer Service and Technical Support Manager | mailto:melg@sapphirebioscience.com

    ________________________

    Sapphire Bioscience Pty. Ltd.

    126 Cope Street │ Waterloo NSW 2017 │ Australia

    Ph: +61 2 9698 2022 │ Fax: +61 2 9698 1022 | http://www.sapphirebioscience.com/

    Read More
    Answer

    Thank you for your enquiry, I am pleased to help.

    From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selection you made:

    ab9110 Rabbit polyclonal tested ICC-IF. This will be suitable

    ab13834 Rabbit polyclonal tested WB not ICC.This one willnot be guaranteed in ICC-IF for their experiments.

    ab27029 - this is conjuated to agarose beads for IP so will not be suitable.

    ab71113 - Rabbit polyclonal tested ICC-IF. This will be suitable.

    Proteinase K:
    Could you confirm for what reason do they need proteinase K? Antigen retrieval is not usually required in ICC and I would suggest to try it only if there is no staining in order to help expose the antigen. In ICC, Fixation should be in just 4% PFA for 10 minutes. Antigen retrieval is usually used for IHC on paraffin embedded sections which are fixed in 10% PFA for at least 24 hours.

    There are many suitable secondaries in the catalog. Here are two exmaples of anti-rabbit secondaries tested in ICC-IF conjugated to FITC as requested by the customer:

    ab97050 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
    Tested in: Flow Cyt, ICC/IF, IHC-P
    https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html).

    ab97199Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (FITC)
    Tested in: Flow Cyt, ICC/IF, IHC-P
    https://www.abcam.com/Goat-Rabbit-IgG-Fc-FITC-ab97199.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-Fc-FITC-ab97199.html).

    We also have an ICC webinar coming soon which they may like to consider reviewing. This is being held next week, regrettably not at a convenient time for Australia. However, a copy will be available on our blog (link at the bottom of the Abcam homepage)in early May.

    The following link aboutthis webinaralso contains links to some ICC protocols (bottom left hand side)which I hope will be helpful:
    https://www.abcam.com/index.html?pageconfig=resource&rid=14548

    I hope this will be useful. If you have any further questions, please do not hesitate to contact me.

    Read More

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